AQP7 Polyclonal antibody

AQP7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 25131-1-AP

Synonyms

AQP 7, AQP7, AQP7L, AQPap, Aquaglyceroporin 7, aquaporin 7, Aquaporin 7 like, Aquaporin adipose

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Validation Data Gallery

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  WB analysis using 25131-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25131-1-AP (AQP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 25131-1-AP

  IP result of anti-AQP7 (IP:25131-1-AP, 4ug; Detection:25131-1-AP 1:300) with mouse kidney tissue lysate 6500ug.

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  IHC staining of human kidney using 25131-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25131-1-AP (AQP7 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 25131-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25131-1-AP (AQP7 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse kidney using 25131-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25131-1-AP (AQP7 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 25131-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25131-1-AP (AQP7 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse testis tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

25131-1-AP targets AQP7 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AQP7 fusion protein Ag17945
• Full Name: aquaporin 7
• Calculated Molecular Weight: 342 aa, 37 kDa
• Observed Molecular Weight: 25-30 kDa, 40 kDa
• GenBank Accession Number: BC062701
• Gene Symbol: AQP7
• Gene ID (NCBI): 364
• RRID: AB_2879913
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AQP7 is a member of the aquaporin family of water-selective membrane channels, localizes to the plasma membrane, and allows movement of water, glycerol and urea across cell membranes. AQP7 forms a channel that mediates water and glycerol transport across cell membranes at neutral pH. AQP7 is highly expressed in the adipose tissue(PMID: 9405233).

Publications

Species	Application	Title
mouse	IF	Gastroenterology; Loss of MYO5B Leads to Reductions in Na+ Absorption With Maintenance of CFTR-Dependent Cl- Secretion in Enterocytes.; Authors - Amy C Engevik
mouse	IF	FASEB J; Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia; Authors - Sheng Gao
mouse	IHC	Biomed Pharmacother; Central administration of Dapagliflozin alleviates a hypothalamic neuroinflammatory signature and changing tubular lipid metabolism in type 2 diabetic nephropathy by upregulating MCPIP1; Authors - Jingjing Da