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  • KD/KO Validated

ARC/ARG3.1 Polyclonal antibody

ARC/ARG3.1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16290-1-AP

Synonyms

ARC, ARC/ARG3.1, Arg3.1, KIAA0278



Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue, rat heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16290-1-AP targets ARC/ARG3.1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARC/ARG3.1 fusion protein Ag9445
Full Name activity-regulated cytoskeleton-associated protein
Calculated molecular weight 396 aa, 45 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC012321
Gene symbol ARC
Gene ID (NCBI) 23237
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration.

Protocols

Product Specific Protocols
WB protocol for ARC/ARG3.1 antibody 16290-1-APDownload protocol
IHC protocol for ARC/ARG3.1 antibody 16290-1-APDownload protocol
IP protocol for ARC/ARG3.1 antibody 16290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Front Cell Neurosci

Herpes Simplex Virus Type 1 Enhances Expression of the Synaptic Protein Arc for Its Own Benefit.

Authors - Francisca Acuña-Hinrichsen
  • KD Validated
mouseIHC

Cell Rep

Experience-Dependent Development and Maintenance of Binocular Neurons in the Mouse Visual Cortex.

Authors - Kyle R Jenks
  • KO Validated
ratWB,IHC

Aging (Albany NY)

EGB761 ameliorates chronic cerebral hypoperfusion-induced cognitive dysfunction and synaptic plasticity impairment.

Authors - Zhao-Hui Yao
humanWB

Aging (Albany NY)

Repressor element-1 silencing transcription factor regulates glutamate receptors and immediate early genes to affect synaptic plasticity.

Authors - Chenhaoyi Xu
mouseWB

Neural Plast

Long-Term Environmental Enrichment Relieves Dysfunctional Cognition and Synaptic Protein Levels Induced by Prenatal Inflammation in Older CD-1 Mice.

Authors - Zhe-Zhe Zhang
mouseWB

Front Neurosci

Dynamic Changes of Cytoskeleton-Related Proteins Within Reward-Related Brain Regions in Morphine-Associated Memory.

Authors - Xixi Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xin (Verified Customer) (01-23-2022)

Although there are some non specific bands, there is a major band at the expected 50 kD.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse cortex lysate
ARC/ARG3.1 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:16290-1-AP)