ARC/ARG3.1 Polyclonal antibody

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  WB analysis of SH-SY5Y using 16290-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 16290-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16290-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 16290-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16290-1-AP

  IP result of anti-ARC/ARG3.1 (IP:16290-1-AP, 4ug; Detection:16290-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 16290-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 16290-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of mouse brain using 16290-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ARC/ARG3.1 antibody (16290-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 16290-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using ARC/ARG3.1 antibody (16290-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

ARC/ARG3.1 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

Cat No. 16290-1-AP

Publications(28)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA

Synonyms

ARC, Activity-regulated cytoskeleton-associated protein, Activity-regulated gene 3.1 protein homolog, Arg3.1, hArc

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse brain tissue, rat heart tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF-Fro detected in: rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 22 publications below
• IHC: See 7 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Product Information

16290-1-AP targets ARC/ARG3.1 in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9445

  Product name: Recombinant human ARC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-396 aa of BC012321

  Sequence: MELDHRTSGGLHAYPGPRGGQVAKPNVILQIGKCRAEMLEHVRRTHRHLLAEVSKQVERELKGLHRSVGKLESNLDGYVPTSDSQRWKKSIKACLCRCQETIANLERWVKREMHVWREVFYRLERWADRLESTGGKYPVGSESARHTVSVGVGGPESYCHEADGYDYTVSPYAITPPPAAGELPGQEPAEAQQYQPWVPGEDGQPSPGVDTQIFEDPREFLSHLEEYLRQVGGSEEYWLSQIQNHMNGPAKKWWEFKQGSVKNWVEFKKEFLQYSEGTLSREAIQRELDLPQKQGEPLDQFLWRKRDLYQTLYVDADEEEIIQYVVGTLQPKLKRFLRHPLPKTLEQLIQRGMEVQDDLEQAAEPAGPHLPVEDEAETLTPAPNSESVASDRTQPE

• Full Name: activity-regulated cytoskeleton-associated protein
• Calculated Molecular Weight: 396 aa, 45 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC012321
• Gene Symbol: ARC
• Gene ID (NCBI): 23237
• RRID: AB_2151832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7LC44
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; PQBP1 promotes translational elongation and regulates hippocampal mGluR-LTD by suppressing eEF2 phosphorylation.; Authors - Yuqian Shen
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
rat	WB,IHC	Aging (Albany NY); EGB761 ameliorates chronic cerebral hypoperfusion-induced cognitive dysfunction and synaptic plasticity impairment.; Authors - Zhao-Hui Yao
human	WB	Aging (Albany NY); Repressor element-1 silencing transcription factor regulates glutamate receptors and immediate early genes to affect synaptic plasticity.; Authors - Chenhaoyi Xu
human	WB	Neurobiol Dis; Prions induce an early Arc response and a subsequent reduction in mGluR5 in the hippocampus.; Authors - Daniel Ojeda-Juarez
mouse	WB,IP,IF	Front Mol Neurosci; Activity-Dependent Arc Expression and Homeostatic Synaptic Plasticity Are Altered in Neurons from a Mouse Model of Angelman Syndrome.; Authors - Elissa D Pastuzyn

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Georgiana (Verified Customer) (01-04-2023)

For the image, magenta is Arc and cyan is GFAP

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain tissue, 20um slices

ARC/ARG3.1 Antibody Immunofluorescence validation (1:500 dilution) in Mouse brain tissue, 20um slices (Cat no:16290-1-AP)

FH

Xin (Verified Customer) (01-23-2022)

Although there are some non specific bands, there is a major band at the expected 50 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

ARC/ARG3.1 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:16290-1-AP)