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  • KD/KO Validated

ARHGAP10 Polyclonal antibody

ARHGAP10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55139-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

ARHGAP10, Graf related protein 2, GRAF2, PS GAP, PSGAP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inmouse heart tissue, HEK-293 cells, human heart tissue, mouse kidney tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55139-1-AP targets ARHGAP10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

fusion protein

Predict reactive species
Full Name Rho GTPase activating protein 10
Calculated Molecular Weight 89 kDa
Observed Molecular Weight 89 kDa
GenBank Accession NumberNM_024605
Gene Symbol ARHGAP10
Gene ID (NCBI) 79658
RRIDAB_10794449
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDA1A4S6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGAP10, also named as GRAF2, is GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. It is essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. ARHGAP10 inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. It stabilizes PAK-2p34 thereby increasing stimulation of cell death. The antibody is specific to ARHGAP10.

Protocols

Product Specific Protocols
WB protocol for ARHGAP10 antibody 55139-1-APDownload protocol
IHC protocol for ARHGAP10 antibody 55139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Metab

Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression

Authors - Lin Qian

Elife

PTEN controls glandular morphogenesis through a juxtamembrane β-Arrestin1/ARHGAP21 scaffolding complex.

Authors - Arman Javadi
humanWB

Mol Ther Nucleic Acids

miR-3174 Contributes to Apoptosis and Autophagic Cell Death Defects in Gastric Cancer Cells by Targeting ARHGAP10.

Authors - Bowen Li
  • KD Validated
mouseWB

Front Cell Dev Biol

The Beta-Tubulin Isotype TUBB6 Controls Microtubule and Actin Dynamics in Osteoclasts.

Authors - Justine Maurin
humanIHC

Front Oncol

Identification and Validation of an Apoptosis-Related Gene Prognostic Signature for Oral Squamous Cell Carcinoma.

Authors - Shuqin Wang
mouseWB

J Biol Chem

The Cdc42 Guanine Nucleotide Exchange Factor FGD6 Coordinates Cell Polarity and Endosomal Membrane Recycling in Osteoclasts.

Authors - Charlotte Steenblock
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