ARHGAP10 Polyclonal antibody

• Featured Product
• KD/KO Validated

ARHGAP10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 55139-1-AP

Synonyms

ARHGAP10, Graf related protein 2, GRAF2, PS GAP, PSGAP

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Validation Data Gallery

• 

  WB analysis of mouse heart using 55139-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55139-1-AP (ARHGAP10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55139-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55139-1-AP (ARHGAP10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 55139-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55139-1-AP (ARHGAP10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 55139-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 55139-1-AP (ARHGAP10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55139-1-AP (ARHGAP10 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55139-1-AP (ARHGAP10 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55139-1-AP (ARHGAP10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55139-1-AP (ARHGAP10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55139-1-AP (ARHGAP10 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55139-1-AP (ARHGAP10 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, HEK-293 cells, human heart tissue, mouse kidney tissue
• Positive IHC detected in: human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 5 publications below

Product Information

55139-1-AP targets ARHGAP10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Rho GTPase activating protein 10
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: NM_024605
• Gene Symbol: ARHGAP10
• Gene ID (NCBI): 79658
• RRID: AB_10794449
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGAP10, also named as GRAF2, is GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. It is essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. ARHGAP10 inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. It stabilizes PAK-2p34 thereby increasing stimulation of cell death. The antibody is specific to ARHGAP10.

Publications

Species	Application	Title
human	WB	Nat Metab; Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression; Authors - Lin Qian
human	WB	Mol Ther Nucleic Acids; miR-3174 Contributes to Apoptosis and Autophagic Cell Death Defects in Gastric Cancer Cells by Targeting ARHGAP10.; Authors - Bowen Li ; KD Validated
mouse	WB	Front Cell Dev Biol; The Beta-Tubulin Isotype TUBB6 Controls Microtubule and Actin Dynamics in Osteoclasts.; Authors - Justine Maurin
human	IHC	Front Oncol; Identification and Validation of an Apoptosis-Related Gene Prognostic Signature for Oral Squamous Cell Carcinoma.; Authors - Shuqin Wang
mouse	WB	J Biol Chem; The Cdc42 Guanine Nucleotide Exchange Factor FGD6 Coordinates Cell Polarity and Endosomal Membrane Recycling in Osteoclasts.; Authors - Charlotte Steenblock
mouse	WB	J Neurol; Rho GTPase-activating protein 10 (ARHGAP10/GRAF2) is a novel autoantibody target in patients with autoimmune encephalitis; Authors - Sven Jarius