GRAF Polyclonal antibody

GRAF Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 17747-1-AP

Synonyms

ARHGAP26, OPHN1L, Oligophrenin-1-like protein, Oligophrenin 1 like protein, KIAA0621

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Validation Data Gallery

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  WB analysis of HeLa using 17747-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 17747-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17747-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17747-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17747-1-AP (GRAF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 17747-1-AP

  IP result of anti-GRAF (IP:17747-1-AP, 4ug; Detection:17747-1-AP 1:1000) with HepG2 cells lysate 3600ug.

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  IHC staining of mouse brain using 17747-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17747-1-AP (GRAF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17747-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17747-1-AP (GRAF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 17747-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17747-1-AP (GRAF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17747-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 17747-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat brain using 17747-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17747-1-AP (GRAF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 17747-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 17747-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17747-1-AP (GRAF antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, human brain tissue, mouse thymus tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: mouse brain tissue, human heart tissue, human liver tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

17747-1-AP targets GRAF in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRAF fusion protein Ag12008
• Full Name: Rho GTPase activating protein 26
• Calculated Molecular Weight: 759 aa, 86 kDa
• Observed Molecular Weight: 92 kDa
• GenBank Accession Number: BC068555
• Gene Symbol: ARHGAP26
• Gene ID (NCBI): 23092
• RRID: AB_2059284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GTPase Regulator Associated with Focal Adhesion Kinase (GRAF), also known as Rho GTPase activating protein 26 (ARHGAP26) , is a GTPase-activating protein and inhibits the activity of Rho GTPases by promoting the hydrolytic ability of Rho GTPases. GRAF enhances the hydrolysis of GTPases and converts GTPases from an active form to an inactive form, thereby inhibiting signaling transduction. Deletion and mutation of GRAF can lead to promyelocytic leukemia, suggesting tumor suppressive activity of GRAF. GRAF was downregulated in glioblastoma and associated with cell proliferation and migration (PMID: 10908648, PMID: 17611651, PMID: 31004081). It has been reported that there are three splicing mutants of GRAF, namely GRAF1a (92 kDa),GRAF1b (86 kDa) and GRAF1c (75-82 kDa), and GRAF1b and GRAF1c are the major GRAF1 isoforms in adult brain, whereas GRAF1a is abundant in neonates(PMID: 35624318, PMID: 30626696).

Publications

Species	Application	Title
mouse	WB	J Neurol; Rho GTPase-activating protein 10 (ARHGAP10/GRAF2) is a novel autoantibody target in patients with autoimmune encephalitis; Authors - Sven Jarius
human	WB	Brain Res; Deficiency of NEAT1 prevented MPP+-induced inflammatory response, oxidative stress and apoptosis in dopaminergic SK-N-SH neuroblastoma cells via miR-1277-5p/ARHGAP26 axis.; Authors - Shufang Zhou
human	WB,IHC,CoIP	Cell Death Dis; CEMIP, acting as a scaffold protein for bridging GRAF1 and MIB1, promotes colorectal cancer metastasis via activating CDC42/MAPK pathway; Authors - Guojie Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cicek (Verified Customer) (06-11-2024)

I used this antibody on mouse neurons, iPSCs and iNeurons with Western blotting, using beta-actin as positive control. There were specific bands at 90 kDa mark for iPSCs and iNeurons, but not for mouse neurons. It was highly expressed in iNeurons. There were unspecific bands at the 70 kDa mark for all samples.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000