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ARL13B Polyclonal antibody

ARL13B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine and More (7)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17711-1-AP

Synonyms

ARL13B, ARL2 like protein 1, ARL2L1, JBTS8



Tested Applications

Positive WB detected inL02 cells, mouse brain tissue, rat liver tissue, NIH/3T3 cells, mouse liver tissue, mouse kidney tissue, rat kidney tissue
Positive IP detected inL02 cells
Positive IHC detected inmouse brain tissue, human testis tissue, human liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells, hTERT-RPE1 cells, hTERT-RPE1 cells, NIH3T3 cells, mouse embryonic fibroblasts

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17711-1-AP targets ARL13B in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman, mouse, rat, canine, chicken, monkey, pig, sheep, Xenopus, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARL13B fusion protein Ag12015
Full Name ADP-ribosylation factor-like 13B
Calculated molecular weight 48 kDa
Observed molecular weight 40-48 kDa, 66 kDa
GenBank accession numberBC094725
Gene symbol ARL13B
Gene ID (NCBI) 200894
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

Protocols

Product Specific Protocols
WB protocol for ARL13B antibody 17711-1-APDownload protocol
IHC protocol for ARL13B antibody 17711-1-APDownload protocol
IF protocol for ARL13B antibody 17711-1-APDownload protocol
IP protocol for ARL13B antibody 17711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Biotechnol

Guided self-organization and cortical plate formation in human brain organoids.

Authors - Madeline A Lancaster
mouseIF

Nature

Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche.

Authors - David Willnow
mouseIF

Science

Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium.

Authors - Avishag Mytlis
humanIF

Nature

A slow-cycling LGR5 tumour population mediates basal cell carcinoma relapse after therapy.

Authors - Adriana Sánchez-Danés
mouseIF

Nature

Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size.

Authors - Matthew B Johnson
humanIF

Cell

Cell Types of the Human Retina and Its Organoids at Single-Cell Resolution.

Authors - Cameron S Cowan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kaviya (Verified Customer) (09-19-2022)

I use this antibody for IHC on brain tissue sections and it generally works well.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:500
FH

Charlotte (Verified Customer) (07-29-2022)

NIH-3T3 stained in DAPI (nucleus), FITC (gamma tubulin), Cy3 (Arl13b). The last picture is the merged channels (FITC and Cy3). On the merged channel, we can conclude that all Cy3 signal is validated by the FITC one (gamma tubulin is present at the base of the primary cilia). Perfect antibody !

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: NIH-3T3
ARL13B Antibody Immunofluorescence validation (1/1000 dilution) in NIH-3T3 (Cat no:17711-1-AP)
FH

Sarah (Verified Customer) (02-16-2021)

Works beautifully in all cell types I've tried. Specifically great to see it work in primary cells (glioblastoma, pictured) as well as iPSCs

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: Primary glioblastoma cells and iPSC
ARL13B Antibody Immunofluorescence validation (1:300 dilution) in Primary glioblastoma cells and iPSC (Cat no:17711-1-AP)
FH

deng (Verified Customer) (01-30-2020)

Very good for cilia staining

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1 : 250
  • Cell Tissue Type: RPE cells
ARL13B Antibody Immunofluorescence, validation (1 : 250 dilution) in RPE cells (Cat no:17711-1-AP)
FH

Gabriela (Verified Customer) (01-01-2020)

Very specific antibody for primary cilia. We use it in chicken embryos spinal cord.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: spinal cord/Chicken
FH

KARTHIKEYAN (Verified Customer) (11-20-2019)

This antibody works really well in human and mouse cell lines and in zebrafish samples, we used this antibody for WB, IF and Flow cytometry.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 for WB and 1:200 for IF
  • Cell Tissue Type: Brain Endothelial cells, brain pericytes, zebrafish embryos
ARL13B Antibody Western Blot,Immunofluorescence, validation (1:1000 for WB and 1:200 for IF dilution) in Brain Endothelial cells, brain pericytes, zebrafish embryos (Cat no:17711-1-AP)
FH

Rebeca (Verified Customer) (09-26-2019)

I have used this antibody for several immunofluorescence stainings of primary cilia. The antibody works beautifully, would definitely recommend!

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse pancreatic organoids
FH

Pavel (Verified Customer) (09-18-2019)

Strong ciliary staining in MDCK cells. Works both with PFA and MeOH fixation.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1/200
  • Cell Tissue Type: MDCK cells
FH

Lindsey (Verified Customer) (02-04-2019)

Excellent specificity with minimal background/nonspecific binding; This antibody is ideal because it works just as well in embryonic mouse tissue as it does in adult. I couldn't recommend this product more!

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: E11.25 Dorsal Root Ganglion/Embryonic Neural Crest Cells
ARL13B Antibody Immunofluorescence, validation (1:1000 dilution) in E11.25 Dorsal Root Ganglion/Embryonic Neural Crest Cells (Cat no:17711-1-AP)
FH

Kishor (Verified Customer) (01-30-2019)

I am very much satisfied with this produced, working good for WB (1:1000) and for IF (1:200)

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 and 1:200
  • Cell Tissue Type: H69, HUCCT-1 and Rat Liver tissue