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- KD/KO Validated
ARNTL Polyclonal antibody
ARNTL Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, CoIP, chIP, ELISA
Cat no : 14268-1-AP
Validation Data Gallery
|Positive WB detected in||HT-1080 cells, HeLa cells, 3T3-L1 cells, MCF-7 cells, NCI-H1299 cells, mouse liver tissue, rat liver tissue, SH-SY5Y cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human prostate cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 6 publications below|
|WB||See 30 publications below|
|IHC||See 10 publications below|
|IF||See 3 publications below|
|CoIP||See 1 publications below|
|ChIP||See 2 publications below|
14268-1-AP targets ARNTL in WB, IP, IHC, IF, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ARNTL fusion protein Ag5586|
|Full Name||aryl hydrocarbon receptor nuclear translocator-like|
|Calculated molecular weight||69 kDa|
|Observed molecular weight||69-75 kDa|
|GenBank accession number||BC041129|
|Gene ID (NCBI)||406|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
BMAL1, a basic helix-loop-helices with Per-Arnt-Sim (PAS) domain transcription factor, is one of components that has core clock function in mammals. It form a heterodimer with CLOCK, and induce the transcription of target genes by binding to E-box elements within their promoter regions. And this transcription is inhibited in a feedback loop by PER, and also by CRY proteins.Also BMAL1 is a key player in the regulation of metabolism, playing a role in adipogenesis and in the control of glucose and triglycerides levels.
|Product Specific Protocols|
|WB protocol for ARNTL antibody 14268-1-AP||Download protocol|
|IHC protocol for ARNTL antibody 14268-1-AP||Download protocol|
|IP protocol for ARNTL antibody 14268-1-AP||Download protocol|
|Click here to view our Standard Protocols|
CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis.
The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.
Circadian Clock Gene Bmal1 Regulates Bilirubin Detoxification: A Potential Mechanism of Feedback Control of Hyperbilirubinemia.
Exogenous melatonin protects preimplantation embryo development from decabromodiphenyl ethane-induced circadian rhythm disorder and endogenous melatonin reduction.
Rev-erbα exacerbates hepatic steatosis in alcoholic liver diseases through regulating autophagy.
Tangeretin prevents cognitive deficit in delirium through activating RORα/γ-E4BP4 axis in mice