BMAL1 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• BMAL1 Polyclonal antibody (14268-1-AP)

• Featured Product
• KD/KO Validated

BMAL1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14268-1-AP Copied!

Publications (123)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA, CUT&Tag

Synonyms

ARNTL, ARNT3, Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic helix-loop-helix ARNT-like protein 1, Basic-helix-loop-helix-PAS protein MOP3

• 

  WB analysis using 14268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14268-1-AP (BMAL1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 14268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14268-1-AP (BMAL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 14268-1-AP

  WB result of BMAL1 antibody (14268-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BMAL1 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of 3T3-L1 using 14268-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 14268-1-AP (ARNTL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HT-1080 using 14268-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14268-1-AP (ARNTL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 14268-1-AP

  IP result of anti-BMAL1 (IP:14268-1-AP, 4ug; Detection:14268-1-AP 1:300) with HeLa cells lysate 1600ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U-87 MG using 14268-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using BMAL1 antibody (14268-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 14268-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARNTL (14268-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for BMAL1 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HT-1080 cells, 3T3-L1 cells, HeLa cells, SH-SY5Y cells, mouse liver tissue, MCF-7 cells, NCI-H1299 cells, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: U-87 MG cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14268-1-AP targets BMAL1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA, CUT&Tag applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5586

  Product name: Recombinant human ARNTL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-303 aa of BC041129

  Sequence: MADQRMDISSTISDFMSPGPTDLLSSSLGTSGVDCNRKRKGSSTDYQESMDTDKDDPHGRLEYTEHQGRIKNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRKLDKLTVLRMAVQHMKTLRGATNPYTEANYKPTFLSDDELKHLILRAADGFLFVVGCDRGKILFVSESVFKILNYSQNDLIGQSLFDYLHPKDIAKVKEQLSSSDTAPRERLIDAKTGLPVKTDITPGPSRLCSGARRSFFCRMKCNRPSVKVEDKDFPSTCSKKKDRKSFCTIHSTGYLKSWPPTKMGLDEDNEP

• Full Name: aryl hydrocarbon receptor nuclear translocator-like
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 69-75 kDa
• GenBank Accession Number: BC041129
• Gene Symbol: ARNTL
• Gene ID (NCBI): 406
• RRID: AB_2878037
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00327
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BMAL1, a basic helix-loop-helices with Per-Arnt-Sim (PAS) domain transcription factor, is one of components that has core clock function in mammals. It form a heterodimer with CLOCK, and induce the transcription of target genes by binding to E-box elements within their promoter regions. And this transcription is inhibited in a feedback loop by PER, and also by CRY proteins.Also BMAL1 is a key player in the regulation of metabolism, playing a role in adipogenesis and in the control of glucose and triglycerides levels. Due to phosphorylation modification, BMAL1 can be detected as a band in the range of 70-80 kda (PMID: 14750952).

Publications

What published studies show

The BMAL1 Polyclonal antibody (Cat# 14268-1-AP) has accumulated 123 citations. Publications appear in high-impact journals including Cell Metabolism, Nature Communications, Molecular Cell, EMBO Journal, Free Radical Biology and Medicine, Cell Death & Disease, Theranostics, and Advanced Science. Research fields span circadian clock biology, cancer, lipid and glucose metabolism, ferroptosis, inflammation, neuroscience, liver disease, bone biology, cardiovascular health, respiratory disorders, sleep disorders, toxicology, and reproductive biology.

Automatically generated from the 123 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	ChIP	Cell Metab; Oncogenic fatty acid oxidation senses circadian disruption in sleep-deficiency-enhanced tumorigenesis; Authors - Fei Peng
mouse	WB,IF,ChIP	Cell Metab; Intestinal clock shapes sleep-wake cycle via sustaining glutamine homeostasis.; Authors - Lianxia Guo
mouse	WB,IHC,IF	Bioact Mater; Bone microenvironment regulative hydrogels with ROS scavenging and prolonged oxygen-generating for enhancing bone repair; Authors - Han Sun ; KD Validated
human	WB	Nat Commun; The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.; Authors - Yi Liu
		Redox Biol; BMAL1 deficiency exacerbates HDM-induced asthma by promoting JUN-mediated lipid peroxidation and airway epithelial ferroptosis.; Authors - Yixin Chen
human	WB	Mol Cell; CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis.; Authors - Ran Lin ; KD Validated