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  • KD/KO Validated

ARNTL Polyclonal antibody

ARNTL Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 14268-1-AP

Synonyms

ARNT3, ARNTL, bHLH PAS protein JAP3, bHLHe5, BMAL1, BMAL1c, Brain and muscle ARNT like 1, JAP3, Member of PAS protein 3, MOP3, PASD3, TIC



Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells, 3T3-L1 cells, MCF-7 cells, NCI-H1299 cells, mouse liver tissue, rat liver tissue, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14268-1-AP targets ARNTL in WB, IP, IHC, IF, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARNTL fusion protein Ag5586
Full Name aryl hydrocarbon receptor nuclear translocator-like
Calculated molecular weight 69 kDa
Observed molecular weight 69-75 kDa
GenBank accession numberBC041129
Gene symbol ARNTL
Gene ID (NCBI) 406
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BMAL1, a basic helix-loop-helices with Per-Arnt-Sim (PAS) domain transcription factor, is one of components that has core clock function in mammals. It form a heterodimer with CLOCK, and induce the transcription of target genes by binding to E-box elements within their promoter regions. And this transcription is inhibited in a feedback loop by PER, and also by CRY proteins.Also BMAL1 is a key player in the regulation of metabolism, playing a role in adipogenesis and in the control of glucose and triglycerides levels.

Protocols

Product Specific Protocols
WB protocol for ARNTL antibody 14268-1-APDownload protocol
IHC protocol for ARNTL antibody 14268-1-APDownload protocol
IP protocol for ARNTL antibody 14268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis.

Authors - Ran Lin
  • KD Validated
humanWB

Nat Commun

The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.

Authors - Yi Liu
mouseIHC

Theranostics

Circadian Clock Gene Bmal1 Regulates Bilirubin Detoxification: A Potential Mechanism of Feedback Control of Hyperbilirubinemia.

Authors - Shuai Wang
mouseWB

Environ Pollut

Exogenous melatonin protects preimplantation embryo development from decabromodiphenyl ethane-induced circadian rhythm disorder and endogenous melatonin reduction.

Authors - Feifei Shi
humanWB

Cell Biosci

Rev-erbα exacerbates hepatic steatosis in alcoholic liver diseases through regulating autophagy.

Authors - Qingxue Liu
mouseWB

Biochem Pharmacol

Tangeretin prevents cognitive deficit in delirium through activating RORα/γ-E4BP4 axis in mice

Authors - Min Chen