ART3 Polyclonal antibody, PBS Only

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  WB analysis using 15930-1-AP (same clone as 15930-1-PBS)

  Various tissues lysates were subjected to SDS PAGE followed by western blot with 15930-1-AP (ART3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15930-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 15930-1-AP (same clone as 15930-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 15930-1-AP (ART3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15930-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 15930-1-AP (same clone as 15930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15930-1-AP (ART3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15930-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 15930-1-AP (same clone as 15930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15930-1-AP (ART3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15930-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ART3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 15930-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ADP ribosyltransferase 3, ADP-ribosyltransferase C2 and C3 toxin-like 3, ARTC3, EC:2.4.2.31, Ecto ADP ribosyltransferase 3

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Product Information

15930-1-PBS targets ART3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8775

  Product name: Recombinant human ART3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 27-364 aa of BC008397

  Sequence: EVLDMADNAFDDEYLKCTDRMEIKYVPQLLKEEKASHQQLDTVWENAKAKWAARKTQIFLPMNFKDNHGIALMAYISEAQEQTPFYHLFSEAVKMAGQSREDYIYGFQFKAFHFYLTRALQLLRKPCEASSKTVVYRTSQGTSFTFGGLNQARFGHFTLAYSAKPQAANDQLTVLSIYTCLGVDIENFLDKESERITLIPLNEVFQVSQEGAGNNLILQSINKTCSHYECAFLGGLKTENCIENLEYFQPIYVYNPGEKNQKLEDHSEKNWKLEDHGEKNQKLEDHGVKILEPTQIPGMKIPEPFPLPEDKSQGNINNPTPGPVPVPGPKSHPSASLG

• Full Name: ADP-ribosyltransferase 3
• Calculated Molecular Weight: 389 aa, 44 kDa
• Observed Molecular Weight: 38-47 kDa
• GenBank Accession Number: BC008397
• Gene Symbol: ART3
• Gene ID (NCBI): 419
• RRID: AB_2060870
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13508
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ART3 is a GPI-anchored mono-ADP-ribosyltransferase enriched on the sperm cell surface, where it participates in spermatid maturation and membrane remodeling. Localized to lipid rafts, ART3 likely modulates cell-cell signaling and extracellular protein modification. Its upregulation in multiple cancers suggests additional roles in migration, invasion, and tumor aggressiveness. Despite incomplete characterization of its enzymatic activity, ART3 remains a key regulator at the interface of reproductive biology, membrane signaling, and pathology.