Anti-Human ART4/CD297 Rabbit Recombinant Antibody

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  FC experiment of Transfected CHO using 98483-1-RR

  1x10^6 CHO cells or ART4/CD297 transfected CHO cells were surface stained with 0.25 ug Anti-Human ART4/CD297 Rabbit RecAb (98483-1-RR, Clone: 250103E6) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  FC experiment of Transfected CHO using 98483-1-RR

  1x10^6 CHO cells or ART4/CD297 transfected CHO cells were surface stained with 0.25 ug Anti-Human ART4/CD297 Rabbit RecAb (98483-1-RR, Clone: 250103E6) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

ART4/CD297 Recombinant Antibody for FC

Cat No. 98483-1-RR

Clone No.250103E6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Ecto ADP ribosyltransferase 4, Mono(ADP ribosyl)transferase 4

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Tested Applications

• Positive FC detected in: Transfected CHO cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98483-1-RR targets ART4/CD297 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ART4/CD297 fusion protein Eg2665
• Full Name: ADP-ribosyltransferase 4 (Dombrock blood group)
• Calculated Molecular Weight: 36 kDa
• GenBank Accession Number: NM_021071.4
• Gene Symbol: ART4
• Gene ID (NCBI): 420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q93070
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Ecto-ADP-ribosyltransferase 4 (ART4), also known as CD297, is a GPI-anchored protein that belongs to the ecto-ADP-ribosyltransferase family. ART4 is the carrier of the Dombrock blood group alloantigens (Do). It is prominently expressed on the surface of erythrocytes and at lower levels also by endothelial cells, monocytes, and the KU-812 leukemia cell line. (PMID: 24478605; 16168396)