ASB9 Polyclonal antibody

ASB9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11952-1-AP

Synonyms

ASB-9, ASB 9

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Validation Data Gallery

• 

  WB analysis of human testis using 11952-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11952-1-AP (ASB9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11952-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11952-1-AP (ASB9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 11952-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11952-1-AP (ASB9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human testis tissue, HepG2 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

11952-1-AP targets ASB9 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASB9 fusion protein Ag2554
• Full Name: ankyrin repeat and SOCS box-containing 9
• Calculated Molecular Weight: 252 aa, 28 kDa, 32 kDa
• Observed Molecular Weight: 28-32 kDa
• GenBank Accession Number: BC001244
• Gene Symbol: ASB9
• Gene ID (NCBI): 140462
• RRID: AB_10666996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Molecules; Identification of Key Candidate Genes Involved in the Progression of Idiopathic Pulmonary Fibrosis.; Authors - Yu Cui
		Oncol Rep; HMQ‑T‑F2 suppresses migration of the human cervical cancer HeLa cells by reversing EMT via the PI3K/Akt signaling pathway.; Authors - Bingling Dai