ASF/SF2 Monoclonal antibody

ASF/SF2 Monoclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1A6C5

Cat no : 66671-1-Ig

Synonyms

Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1



Tested Applications

Positive WB detected inHeLa cells, LNCaP cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66671-1-Ig targets ASF/SF2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASF/SF2 fusion protein Ag3626
Full Name splicing factor, arginine/serine-rich 1
Calculated molecular weight 248 aa, 28 kDa
Observed molecular weight 32 kDa, 28 kDa, 22 kDa
GenBank accession numberBC010264
Gene symbol SFRS1
Gene ID (NCBI) 6426
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.

Protocols

Product Specific Protocols
WB protocol for ASF/SF2 antibody 66671-1-IgDownload protocol
IHC protocol for ASF/SF2 antibody 66671-1-IgDownload protocol
IF protocol for ASF/SF2 antibody 66671-1-IgDownload protocol
IP protocol for ASF/SF2 antibody 66671-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols