Filter: AllWBIPIHCIF

WB analysis using 66671-1-Ig (same clone as 66671-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.
WB analysis using 66671-1-Ig (same clone as 66671-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 66671-1-Ig (same clone as 66671-1-PBS)
IP result of anti-ASF/SF2 (IP:66671-1-Ig, 5ug; Detection:66671-1-Ig 1:5000) with HeLa cells lysate 2200 ug. This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 66671-1-Ig (same clone as 66671-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66671-1-Ig (same clone as 66671-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66671-1-Ig (same clone as 66671-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 66671-1-Ig (same clone as 66671-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66671-1-Ig (same clone as 66671-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ASF/SF2 antibody (66671-1-Ig, Clone: 1A6C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66671-1-Ig (same clone as 66671-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

ASF/SF2 Monoclonal antibody, PBS Only

ASF/SF2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 66671-1-PBS

Clone No.1A6C5

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66671-1-PBS targets ASF/SF2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag3626 Product name: Recombinant human ASF/SF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-248 aa of BC010264 Sequence: MSGGGVIRGPAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGTGRGGGGGGGGGAPRGRYGPPSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRSRSRSRSRSRSNSRSRSYSPRRSRGSPRYSPRHSRSRSRT Predict reactive species
Full Name	splicing factor, arginine/serine-rich 1
Calculated Molecular Weight	248 aa, 28 kDa
Observed Molecular Weight	32 kDa, 28 kDa, 22 kDa
GenBank Accession Number	BC010264
Gene Symbol	ASF/SF2
Gene ID (NCBI)	6426
RRID	AB_2882025
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q07955
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.