CoraLite® Plus 488-conjugated ASF/SF2 Monoclonal antibody

ASF/SF2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1


Human, mouse, rat




CoraLite® Plus 488 Fluorescent Dye



Cat no : CL488-66671


Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1

Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66671 targets ASF/SF2 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASF/SF2 fusion protein Ag3626
Full Name splicing factor, arginine/serine-rich 1
Calculated molecular weight 248 aa, 28 kDa
Observed molecular weight 32 kDa, 28 kDa, 22 kDa
GenBank accession numberBC010264
Gene symbol SFRS1
Gene ID (NCBI) 6426
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.


Product Specific Protocols
IF protocol for CL Plus 488 ASF/SF2 antibody CL488-66671Download protocol
Standard Protocols
Click here to view our Standard Protocols