CoraLite®488-conjugated ASGR1 Polyclonal antibody

ASGR1 Polyclonal Antibody for FC, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

Cat no : CL488-11739

Synonyms

ASGP R 1, ASGPR, ASGPR 1, ASGR1, asialoglycoprotein receptor 1, CLEC4H1, Hepatic lectin H1, HL 1, Hs.12056



Tested Applications

Positive IF detected inhuman liver cancer tissue
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 11739-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-11739 targets ASGR1 in IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASGR1 fusion protein Ag2310
Full Name asialoglycoprotein receptor 1
Calculated molecular weight 291 aa, 33 kDa
GenBank accession numberBC032130
Gene symbol ASGR1
Gene ID (NCBI) 432
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated ASGR1 antibody CL488-11739Download protocol
FC protocol for CL488-conjugated ASGR1 antibody CL488-11739Download protocol
Standard Protocols
Click here to view our Standard Protocols