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ASGR1 Polyclonal antibody

ASGR1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11739-1-AP

Synonyms

ASGP R 1, ASGPR, ASGPR 1, Asgpr1, ASGR1, asialoglycoprotein receptor 1, CLEC4H1, Hepatic lectin H1, HL 1, Hs.12056



Tested Applications

Positive WB detected inHepG2 cells, human liver tissue, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inrat liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue, rat liver tissue
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11739-1-AP targets ASGR1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASGR1 fusion protein Ag2310
Full Name asialoglycoprotein receptor 1
Calculated molecular weight 291 aa, 33 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC032130
Gene symbol ASGR1
Gene ID (NCBI) 432
RRIDAB_2059675
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

Protocols

Product Specific Protocols
WB protocol for ASGR1 antibody 11739-1-APDownload protocol
IHC protocol for ASGR1 antibody 11739-1-APDownload protocol
IF protocol for ASGR1 antibody 11739-1-APDownload protocol
IP protocol for ASGR1 antibody 11739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

Nature

Regulated control of gene therapies by drug-induced splicing.

Authors - Alex Mas Monteys
mouseFC

Nano Lett

A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo.

Authors - Khalid A Hajj
humanIF

Acta Biomater

Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply.

Authors - Kun Du
humanWB

Biomaterials

Dual targeted delivery of statins and nucleic acids by chitosan-based nanoparticles for enhanced antiatherosclerotic efficacy.

Authors - Ting Jiang
humanWB

Cancer Res

ASO-Based PKM Splice-Switching Therapy Inhibits Hepatocellular Carcinoma Growth.

Authors - Wai Kit Ma
humanIHC,IF

Mol Ther

Enhanced Potency of GalNAc-Conjugated Antisense Oligonucleotides in Hepatocellular Cancer Models.

Authors - Youngsoo Kim

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:25000 incubated at 4 degree C overnight. ASGR1 bands appeared at about 42 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:25000
  • Cell Tissue Type: Mouse liver tissue
FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:25000 incubated at 4 degree C overnight. ASGR1 bands appeared at about 42 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:25000
  • Cell Tissue Type: Mouse liver tissue
FH

James (Verified Customer) (11-18-2022)

Very good antibody! Used it to label hepatocytes in section of paraffin-embedded liver tissue

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Liver
ASGR1 Antibody Immunofluorescence validation (1/1000 dilution) in Liver (Cat no:11739-1-AP)