Validation Data Gallery
|Positive WB detected in||mouse liver tissue, human liver tissue, rat liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human liver cancer tissue|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11739-1-AP targets ASGR1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ASGR1 fusion protein Ag2310|
|Full Name||asialoglycoprotein receptor 1|
|Calculated molecular weight||291 aa, 33 kDa|
|Observed molecular weight||40 kDa|
|GenBank accession number||BC032130|
|Gene ID (NCBI)||432|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.
|Product Specific Protocols|
|WB protocol for ASGR1 antibody 11739-1-AP||Download protocol|
|IHC protocol for ASGR1 antibody 11739-1-AP||Download protocol|
|IF protocol for ASGR1 antibody 11739-1-AP||Download protocol|
|IP protocol for ASGR1 antibody 11739-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mol Ther Nucleic Acids
Delivery of GalNAc-Conjugated Splice-Switching ASOs to Non-hepatic Cells through Ectopic Expression of Asialoglycoprotein Receptor.
Characterization of Cellular Sources and Circulating Levels of Extracellular Vesicles in a Dietary Murine Model of Nonalcoholic Steatohepatitis.
Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply.
Phosphatidylserine-deficient small extracellular vesicle is a major somatic cell-derived sEV subpopulation in blood.
J Am Heart Assoc
Neuraminidases 1 and 3 Trigger Atherosclerosis by Desialylating Low-Density Lipoproteins and Increasing Their Uptake by Macrophages.
Hum Mol Genet
The FUT2 secretor variant p.Trp154Ter influences serum vitamin B12 concentration via holo-haptocorrin, but not holo-transcobalamin, and is associated with haptocorrin glycosylation.