ASNA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ASNA1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15450-1-AP

Synonyms

ARSA, ARSA I, ARSA1, ASNA1, ATPase ASNA1, hARSA I, hASNA I, TRC40

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Validation Data Gallery

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  WB analysis of human brain using 15450-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15450-1-AP (ASNA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15450-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15450-1-AP (ASNA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15450-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15450-1-AP (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15450-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15450-1-AP (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15450-1-AP

  IP result of anti-ASNA1 (IP:15450-1-AP, 4ug; Detection:15450-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lung cancer using 15450-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15450-1-AP (ASNA1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 15450-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15450-1-AP (ASNA1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 15450-1-AP

  Immunofluorescent analysis of HepG2 cells, using ASNA1 antibody 15450-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 15450-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug ASNA1 antibody (15450-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: human brain tissue, HeLa cells, human heart tissue, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below

Product Information

15450-1-AP targets ASNA1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASNA1 fusion protein Ag7713
• Full Name: arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
• Calculated Molecular Weight: 38.7 kDa
• Observed Molecular Weight: 37-41 kDa
• GenBank Accession Number: BC002651
• Gene Symbol: ASNA1
• Gene ID (NCBI): 439
• RRID: AB_2060110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to DDP and arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.

Publications

Species	Application	Title
human	WB	Mol Cell; Molecular machinery for insertion of tail-anchored membrane proteins into the endoplasmic reticulum membrane in mammalian cells.; Authors - Yasunori Yamamoto
human	WB	J Cell Sci; Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.; Authors - Janine Pfaff ; KD Validated
human	WB	J Cell Sci; An alternative membrane topology permits lipid droplet localization of peroxisomal fatty acyl-CoA reductase 1.; Authors - Tarik Exner ; KD Validated
human	WB	J Cell Sci; A trap mutant reveals the physiological client spectrum of TRC40.; Authors - Javier Coy-Vergara ; KD Validated
human	WB	J Biol Chem; ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence; Authors - Tetsuya Hirata
human	WB	FEBS Lett; Human SND2 mediates ER targeting of GPI-anchored proteins with low hydrophobic GPI attachment signals.; Authors - Jing Yang