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  • KD/KO Validated

ASNA1 Polyclonal antibody

ASNA1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15450-1-AP

Synonyms

ARSA, ARSA I, ARSA1, ASNA1, ATPase ASNA1, hARSA I, hASNA I, TRC40



Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells, human heart tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15450-1-AP targets ASNA1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASNA1 fusion protein Ag7713
Full Name arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
Calculated molecular weight 38.7 kDa
Observed molecular weight 37-41 kDa
GenBank accession numberBC002651
Gene symbol ASNA1
Gene ID (NCBI) 439
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to DDP and arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.

Protocols

Product Specific Protocols
WB protocol for ASNA1 antibody 15450-1-APDownload protocol
IHC protocol for ASNA1 antibody 15450-1-APDownload protocol
IF protocol for ASNA1 antibody 15450-1-APDownload protocol
IP protocol for ASNA1 antibody 15450-1-APDownload protocol
FC protocol for ASNA1 antibody 15450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Sci

A trap mutant reveals the physiological client spectrum of TRC40.

Authors - Javier Coy-Vergara
  • KD Validated
humanWB

J Cell Sci

Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.

Authors - Janine Pfaff
  • KD Validated
humanWB

Mol Cell

Molecular machinery for insertion of tail-anchored membrane proteins into the endoplasmic reticulum membrane in mammalian cells.

Authors - Yasunori Yamamoto