|Positive WB detected in||human brain tissue, HeLa cells, human heart tissue, mouse brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15450-1-AP targets ASNA1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ASNA1 fusion protein Ag7713|
|Full Name||arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)|
|Calculated molecular weight||38.7 kDa|
|Observed molecular weight||37-41 kDa|
|GenBank accession number||BC002651|
|Gene ID (NCBI)||439|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to DDP and arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.
|Product Specific Protocols|
|WB protocol for ASNA1 antibody 15450-1-AP||Download protocol|
|IHC protocol for ASNA1 antibody 15450-1-AP||Download protocol|
|IF protocol for ASNA1 antibody 15450-1-AP||Download protocol|
|IP protocol for ASNA1 antibody 15450-1-AP||Download protocol|
|FC protocol for ASNA1 antibody 15450-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Cell Sci
A trap mutant reveals the physiological client spectrum of TRC40.
J Cell Sci
Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.
Molecular machinery for insertion of tail-anchored membrane proteins into the endoplasmic reticulum membrane in mammalian cells.