|Positive WB detected in||pig liver tissue, HeLa cells, human liver tissue, L02 cells|
|Positive IHC detected in||human liver tissue, human liver cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66036-1-Ig is ASS1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||ASS1 fusion protein Ag9314|
|Full Name||argininosuccinate synthetase 1|
|Calculated molecular weight||412 aa, 47 kDa|
|Observed molecular weight||40-45 kDa|
|GenBank accession number||BC009243|
|Gene ID (NCBI)||445|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).
Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy.
Argininosuccinate synthetase 1 contributes to gastric cancer invasion and progression by modulating autophagy.