Filter: AllWBIHCIF/ICC

WB analysis of pig liver using 66036-1-Ig (same clone as 66036-1-PBS)
pig liver tissue were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.
WB analysis of L02 using 66036-1-Ig (same clone as 66036-1-PBS)
L02 cells were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.
WB analysis of human liver using 66036-1-Ig (same clone as 66036-1-PBS)
human liver tissue were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66036-1-Ig (same clone as 66036-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66036-1-Ig (same clone as 66036-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66036-1-Ig (same clone as 66036-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66036-1-Ig (same clone as 66036-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66036-1-Ig (same clone as 66036-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 66036-1-Ig (same clone as 66036-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 66036-1-Ig (same clone as 66036-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66036-1-Ig (same clone as 66036-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66036-1-Ig (same clone as 66036-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 66036-1-Ig (same clone as 66036-1-PBS)
Immunofluorescent analysis of MCF-7 cells, using ASS1 antibody 66036-1-Ig at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

ASS1 Monoclonal antibody, PBS Only

ASS1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66036-1-PBS

Clone No.1D1A11

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1D1A11, ASS, EC:6.3.4.5

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66036-1-PBS targets ASS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity	human, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag9314 Product name: Recombinant human ASS1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 149-412 aa of BC009243 Sequence: EFYNRFKGRNDLMEYAKQHGIPIPVTPKNPWSMDENLMHISYEAGILENPKNQAPPGLYTKTQDPAKAPNTPDILEIEFKKGVPVKVTNVKDGTTHQTSLELFMYLNEVAGKHGVGRIDIVENRFIGMKSRGIYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIAKSQERVEGKVQVSVLKGQVYILGRESPLSLYNEELVSMNVQGDYEPTDATGFININSLRLKEYHRLQSKVTAK Predict reactive species
Full Name	argininosuccinate synthetase 1
Calculated Molecular Weight	412 aa, 47 kDa
Observed Molecular Weight	40-45 kDa
GenBank Accession Number	BC009243
Gene Symbol	ASS1
Gene ID (NCBI)	445
RRID	AB_11042883
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P00966
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).