ATF7IP Polyclonal antibody

• Featured Product
• KD/KO Validated

ATF7IP Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14699-1-AP

Synonyms

ATF7IP, MCAF, p621

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Validation Data Gallery

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  IHC staining of human breast cancer using 14699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 14699-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 14699-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

14699-1-AP targets ATF7IP in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATF7IP fusion protein Ag6341
• Full Name: activating transcription factor 7 interacting protein
• Calculated Molecular Weight: 137 kDa
• GenBank Accession Number: BC063855
• Gene Symbol: ATF7IP
• Gene ID (NCBI): 55729
• RRID: AB_2878074
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Hum Mol Genet; Mechanism suppressing H3K9 trimethylation in pluripotent stem cells and its demise by polyQ-expanded huntingtin mutations.; Authors - Dilber Irmak ; KD Validated

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