ATG14/Barkor Polyclonal antibody

ATG14/Barkor Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 28021-1-AP

Synonyms

ATG14, Atg14L, Barkor, KIAA0831

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Validation Data Gallery

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  WB analysis using 28021-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28021-1-AP (ATG14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 28021-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 28021-1-AP (ATG14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 28021-1-AP

  IP result of anti-ATG14/Barkor (IP:28021-1-AP, 4ug; Detection:28021-1-AP 1:500) with rat brain tissue lysate 4000 ug.

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  IHC staining of human liver cancer using 28021-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28021-1-AP (ATG14 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28021-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28021-1-AP (ATG14 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, A549 cells, rat brain tissue
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 1 publications below

Product Information

28021-1-AP targets ATG14/Barkor in WB, IP, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG14/Barkor fusion protein Ag27671
• Full Name: KIAA0831
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 and 65 kDa
• GenBank Accession Number: NM_014924
• Gene Symbol: KIAA0831
• Gene ID (NCBI): 22863
• RRID: AB_2881038
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG14 plays a critical role in autophagy which is required for both basal and inducible autophagy. It has been reported that the loss of ATG14 completely blocks autophagic fusion with lysosomes. ATG14 is associated with the location of PI3-kinase complex PI3KC3-C1 and the formation of autophagosome. Besides, BECN2 can form the BECN2:ATG14 heterodimer with ATG14. 28021-1-AP antibody recognizes the 55 kDa protein and the phosphorylated 65 kDa protein in SDS-PAGE. (PMID: 28218432, 24597603, 30894050)

Publications

Species	Application	Title
human	WB	Cancer Lett; Targeting PP2A with lomitapide suppresses colorectal tumorigenesis through the activation of AMPK/Beclin1-mediated autophagy.; Authors - Qian Zuo
human	WB	Ecotoxicol Environ Saf; Weakened interaction of ATG14 and the SNARE complex blocks autophagosome-lysosome fusion contributes to fluoride-induced developmental neurotoxicity.; Authors - Yuanli Zhang
mouse	WB	Cells; Cytosolic HMGB1 Mediates LPS-Induced Autophagy in Microglia by Interacting with NOD2 and Suppresses Its Proinflammatory Function; Authors - Seung-Woo Kim
human	WB	Life (Basel); MiR-371a-5p Positively Associates with Hepatocellular Carcinoma Malignancy but Sensitizes Cancer Cells to Oxaliplatin by Suppressing BECN1-Dependent Autophagy; Authors - Zhenbing Lv
mouse	WB	FASEB J; Melatonin ameliorates cerebral ischemia-reperfusion injury in diabetic mice by enhancing autophagy via the SIRT1-BMAL1 pathway; Authors - Lian Liu
human	WB,IHC	Heliyon; Elevated GABRP expression is correlated to the excessive autophagy in intrahepatic cholestasis of pregnancy; Authors - Ruihong Dong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Balawant (Verified Customer) (12-25-2023)

I have used this antibody, and it is working great. this antibody has specific western blot band and no background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mice colon tissue and colon cancer cell line