ATG14/Barkor Polyclonal antibody

ATG14/Barkor Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28021-1-AP

Synonyms

ATG14, Atg14L, Barkor, KIAA0831



Tested Applications

Positive WB detected inmouse brain tissue, A549 cells, rat brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

28021-1-AP targets ATG14/Barkor in WB, IP, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG14/Barkor fusion protein Ag27671
Full Name KIAA0831
Calculated molecular weight 55 kDa
Observed molecular weight 55 and 65 kDa
GenBank accession numberNM_014924
Gene symbol KIAA0831
Gene ID (NCBI) 22863
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG14 plays a critical role in autophagy which is required for both basal and inducible autophagy. It has been reported that the loss of ATG14 completely blocks autophagic fusion with lysosomes. ATG14 is associated with the location of PI3-kinase complex PI3KC3-C1 and the formation of autophagosome. Besides, BECN2 can form the BECN2:ATG14 heterodimer with ATG14. 28021-1-AP antibody recognizes the 55 kDa protein and the phosphorylated 65 kDa protein in SDS-PAGE. (PMID: 28218432, 24597603, 30894050)

Protocols

Product Specific Protocols
WB protocol for ATG14/Barkor antibody 28021-1-APDownload protocol
IHC protocol for ATG14/Barkor antibody 28021-1-APDownload protocol
IP protocol for ATG14/Barkor antibody 28021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Lett

Targeting PP2A with lomitapide suppresses colorectal tumorigenesis through the activation of AMPK/Beclin1-mediated autophagy.

Authors - Qian Zuo
humanWB

Ecotoxicol Environ Saf

Weakened interaction of ATG14 and the SNARE complex blocks autophagosome-lysosome fusion contributes to fluoride-induced developmental neurotoxicity.

Authors - Yuanli Zhang
mouseWB

Cells

Cytosolic HMGB1 Mediates LPS-Induced Autophagy in Microglia by Interacting with NOD2 and Suppresses Its Proinflammatory Function

Authors - Seung-Woo Kim
humanWB

Life (Basel)

MiR-371a-5p Positively Associates with Hepatocellular Carcinoma Malignancy but Sensitizes Cancer Cells to Oxaliplatin by Suppressing BECN1-Dependent Autophagy

Authors - Zhenbing Lv
mouseWB

FASEB J

Melatonin ameliorates cerebral ischemia-reperfusion injury in diabetic mice by enhancing autophagy via the SIRT1-BMAL1 pathway

Authors - Lian Liu
humanWB,IHC

Heliyon

Elevated GABRP expression is correlated to the excessive autophagy in intrahepatic cholestasis of pregnancy

Authors - Ruihong Dong