ATG14 Polyclonal antibody

ATG14 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 28021-1-AP

Synonyms

ATG14, Atg14L, Barkor, KIAA0831



Tested Applications

Positive WB detected inmouse brain tissue, A549 cells, rat brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

28021-1-AP targets ATG14 in WB, IP, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG14 fusion protein Ag27671
Full Name KIAA0831
Calculated molecular weight 55 kDa
Observed molecular weight 55 and 65 kDa
GenBank accession numberNM_014924
Gene symbol KIAA0831
Gene ID (NCBI) 22863
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATG14 plays a critical role in autophagy which is required for both basal and inducible autophagy. It has been reported that the loss of ATG14 completely blocks autophagic fusion with lysosomes. ATG14 is associated with the location of PI3-kinase complex PI3KC3-C1 and the formation of autophagosome. Besides, BECN2 can form the BECN2:ATG14 heterodimer with ATG14. 28021-1-AP antibody recognizes the 55 kDa protein and the phosphorylated 65 kDa protein in SDS-PAGE. (PMID: 28218432, 24597603, 30894050)

Protocols

Product Specific Protocols
WB protocol for ATG14 antibody 28021-1-APDownload protocol
IHC protocol for ATG14 antibody 28021-1-APDownload protocol
IP protocol for ATG14 antibody 28021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols