ATG3 Polyclonal antibody

ATG3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 11262-2-AP


APG3, APG3 LIKE, APG3L, ATG3, Autophagy related protein 3, hApg3, PC3 96, Protein PC3 96

Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, Jurkat cells, K-562 cells, THP-1 cells, PC-12 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11262-2-AP targets ATG3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG3 fusion protein Ag1785
Full Name ATG3 autophagy related 3 homolog (S. cerevisiae)
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36-40 kDa
GenBank Accession NumberBC024221
Gene Symbol ATG3
Gene ID (NCBI) 64422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Autophagy is a lysosome-dependent conserved catabolic process in response to various stresses, such as oxidative stress and ischemia, for the degradation and recycling of aged or dysfunctional intracellular components and damaged organelles. A large group of autophagy-related genes (ATGs) have been identifed as essential drivers in diferent stages of autophagy. Autophagy-related 3 (ATG3), one of the autophagyrelated gene family members, serves as an E2-like enzyme contributing to the conjugation of microtubule-associated protein light chain 3 (LC3) to lipid phosphotidylethanolamine (PE). ATG3 plays a signifcant role in the regulation of autophagy, viability, and death.


Product Specific Protocols
WB protocol for ATG3 antibody 11262-2-APDownload protocol
IHC protocol for ATG3 antibody 11262-2-APDownload protocol
IF protocol for ATG3 antibody 11262-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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