ATG3 Polyclonal antibody

ATG3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11262-2-AP

Synonyms

APG3, APG3 LIKE, APG3L, ATG3, Autophagy related protein 3, hApg3, PC3 96, Protein PC3 96



Tested Applications

Positive WB detected inHL-60 cells, mouse kidney tissue, K-562 cells, PC-12 cells, THP-1 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11262-2-AP targets ATG3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG3 fusion protein Ag1785
Full Name ATG3 autophagy related 3 homolog (S. cerevisiae)
Calculated molecular weight 36 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC024221
Gene symbol ATG3
Gene ID (NCBI) 64422
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATG3 antibody 11262-2-APDownload protocol
IHC protocol for ATG3 antibody 11262-2-APDownload protocol
IF protocol for ATG3 antibody 11262-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Stem Cell Rev Rep

Activation of Autophagy Ameliorates Age-Related Neurogenesis Decline and Neurodysfunction in Adult Mice.

Authors - Na Yang
humanIHC

Cell Death Dis

Eukaryotic initiation factor 5A2 mediates hypoxia-induced autophagy and cisplatin resistance.

Authors - Guodong Xu
mouseWB

Int J Ophthalmol

Autophagy activation and the mechanism of retinal microvascular endothelial cells in hypoxia.

Authors - Rong Li
ratWB

Nutr Metab (Lond)

Resveratrol promotes osteoblastic differentiation in a rat model of postmenopausal osteoporosis by regulating autophagy.

Authors - Wei Wang
humanWB

Mar Drugs

7-Acetylsinumaximol B Induces Apoptosis and Autophagy in Human Gastric Carcinoma Cells through Mitochondria Dysfunction and Activation of the PERK/eIF2α/ATF4/CHOP Signaling Pathway.

Authors - Tsung-Chang Tsai
mouseWB

Food Funct

EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.

Authors - Jian Fang