ATG3 Polyclonal antibody

ATG3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11262-2-AP

Synonyms

APG3, APG3 LIKE, APG3L, ATG3, Autophagy related protein 3, hApg3, PC3 96, Protein PC3 96



Tested Applications

Positive WB detected inHL-60 cells, mouse kidney tissue, K-562 cells, PC-12 cells, THP-1 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11262-2-AP targets ATG3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG3 fusion protein Ag1785
Full Name ATG3 autophagy related 3 homolog (S. cerevisiae)
Calculated molecular weight 36 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC024221
Gene symbol ATG3
Gene ID (NCBI) 64422
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATG3 antibody 11262-2-APDownload protocol
IHC protocol for ATG3 antibody 11262-2-APDownload protocol
IF protocol for ATG3 antibody 11262-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy.

Authors - Bo Wu
pigWB

Chemosphere

Genome-wide transcriptional profiling and functional analysis reveal miR-330-MAPK15 axis involving in cellular responses to deoxynivalenol exposure.

Authors - Haifei Wang
mouseWB

Front Cell Dev Biol

Aquaporin-8 transports hydrogen peroxide to regulate granulosa cell autophagy

Authors - Binbin Huang
mouseWB

Front Immunol

Toxoplasma gondii CDPK3 Controls the Intracellular Proliferation of Parasites in Macrophages.

Authors - Minmin Wu
humanWB

Oncotarget

Upregulation of the lncRNA Meg3 induces autophagy to inhibit tumorigenesis and progression of epithelial ovarian carcinoma by regulating activity of ATG3.

Authors - Yin-Ling Xiu
mouseWB

Ecotoxicol Environ Saf

Exposure to DEHP induces testis toxicity and injury through the ROS/mTOR/NLRP3 signaling pathway in immature rats.

Authors - Yifan Hong