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ATM Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 27156-1-AP

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Con: 50 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

ATM Antibody WB HEK-293 cells 27156-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 27156-1-AP( ATM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

ATM Antibody IHC human breast cancer tissue 27156-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ATM Antibody IHC human breast cancer tissue 27156-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ATM Antibody IF HeLa cells 27156-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using 27156-1-AP (ATM antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse
Positive WB detected in:
HEK-293 cells, HepG2 cells, HeLa cells
Positive IHC detected in:
human breast cancer tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ATM fusion protein Ag25909
Full name:
ataxia telangiectasia mutated
Calculated molecular weight:
Observed molecular weight:
350 kDa
GenBank accession number:
BC137169
Gene ID (NCBI):
472
Gene symbol
ATM
Synonyms
A T mutated, AT1, ATA, ataxia telangiectasia mutated, ATC, ATD, ATDC, ATE, ATM, Serine protein kinase ATM, TEL1, TELO1

Background

The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.


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