ATM Polyclonal antibody

ATM Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27156-1-AP

Synonyms

A T mutated, AT1, ATA, ataxia telangiectasia mutated, ATC, ATD, ATDC, ATE, ATM, Serine protein kinase ATM, TEL1, TELO1



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 7 publications below

Product Information

The immunogen of 27156-1-AP is ATM Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATM fusion protein Ag25909
Full Name ataxia telangiectasia mutated
Observed molecular weight 310 kDa
GenBank accession numberBC137169
Gene symbol ATM
Gene ID (NCBI) 472
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.

Protocols

Product Specific Protocols
WB protocol for ATM antibody 27156-1-APDownload protocol
IHC protocol for ATM antibody 27156-1-APDownload protocol
IF protocol for ATM antibody 27156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Br J Pharmacol

Potent effects of dioscin against hepatocellular carcinoma through regulating TIGAR-mediated apoptosis, autophagy and DNA damage.

Authors - Zhang Mao
humanWB

J Cell Mol Med

Potent USP10/13 antagonist spautin-1 suppresses melanoma growth via ROS-mediated DNA damage and exhibits synergy with cisplatin.

Authors - Jia Guo
mouseWB

Plant Foods Hum Nutr

Anthocyanins Delay Ageing-Related Degenerative Changes in the Liver.

Authors - Jie Wei
mouseWB

J Agric Food Chem

Anthocyanins from Black Chokeberry (Aroniamelanocarpa Elliot) Delayed Aging-Related Degenerative Changes of Brain.

Authors - Jie Wei
humanWB

Med Oncol

PMID: 31372848

Authors - Jingrui Jin
humanWB

Cell Prolif

RNF8 promotes efficient DSB repair by inhibiting the pro-apoptotic activity of p53 through regulating the function of Tip60.

Authors - Hongyu Chen