ATM Polyclonal antibody

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  WB analysis using 27156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27156-1-AP (ATM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 27156-1-AP

  WB result of ATM antibody (27156-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and sg-ATM transfected A549 cells.

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  WB analysis of HEK-293 using 27156-1-AP

  WB result of ATM antibody (27156-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATM transfected HEK-293 cells.

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  IP experiment of HEK-293 using 27156-1-AP

  IP result of anti-ATM (IP:27156-1-AP, 4ug; Detection:27156-1-AP 1:800) with HEK-293 cells lysate 1480 ug.

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  IHC staining of human breast cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

ATM Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 27156-1-AP

Publications(41)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Synonyms

Serine-protein kinase ATM, EC:2.7.11.1, A-T mutated, A T mutated

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 40 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Product Information

27156-1-AP targets ATM in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATM fusion protein Ag25909
• Full Name: ataxia telangiectasia mutated
• Observed Molecular Weight: 310-350 kDa
• GenBank Accession Number: BC137169
• Gene Symbol: ATM
• Gene ID (NCBI): 472
• RRID: AB_2880780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13315
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.

Publications

Species	Application	Title
human	WB	Sci Transl Med; Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer; Authors - Jia-Han Ding
mouse	WB	Acta Pharm Sin B; Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis; Authors - Yujun Zhou
human	WB	Cell Death Differ; p53-dependent elimination of aneuploid mitotic offspring by entosis.; Authors - Jianqing Liang
human	WB	Oncogene; TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex; Authors - Ya Wang
	WB	Clin Transl Med; Single-cell profiling-guided combination therapy of c-Fos and histone deacetylase inhibitors in diffuse large B-cell lymphoma.; Authors - Luqiao Wang
human	WB	EBioMedicine; MAP4K1 functions as a tumor promotor and drug mediator for AML via modulation of DNA damage/repair system and MAPK pathway.; Authors - Qing Ling