ATP1B3 Monoclonal antibody

• Featured Product
• KD/KO Validated

ATP1B3 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1H9C1

Cat no : 67554-1-Ig

Synonyms

ATP1B3, ATPB 3, CD298, FLJ29027

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Validation Data Gallery

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  WB analysis of Jurkat using 67554-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 67554-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67554-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67554-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67554-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67554-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67554-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67554-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67554-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells, human placenta tissue, THP-1 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• IHC: See 1 publications below

Product Information

67554-1-Ig targets ATP1B3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP1B3 fusion protein Ag30127
• Full Name: ATPase, Na+/K+ transporting, beta 3 polypeptide
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 38-43 kDa
• GenBank Accession Number: BC011835
• Gene Symbol: ATP1B3
• Gene ID (NCBI): 483
• RRID: AB_2882768
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).

Publications

Species	Application	Title
human	IHC	Front Immunol; Integrative Transcriptomic, Proteomic and Functional Analysis Reveals ATP1B3 as a Diagnostic and Potential Therapeutic Target in Hepatocellular Carcinoma.; Authors - Shanshan Lu ; KD Validated