ATP1B3 Monoclonal antibody

ATP1B3 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1H9C1

Cat no : 67554-1-Ig

Synonyms

ATP1B3, ATPB 3, CD298, FLJ29027



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, human placenta tissue, THP-1 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67554-1-Ig targets ATP1B3 in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP1B3 fusion protein Ag30127
Full Name ATPase, Na+/K+ transporting, beta 3 polypeptide
Calculated molecular weight 32 kDa
Observed molecular weight 38-43 kDa
GenBank accession numberBC011835
Gene symbol ATP1B3
Gene ID (NCBI) 483
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).

Protocols

Product Specific Protocols
WB protocol for ATP1B3 antibody 67554-1-IgDownload protocol
IHC protocol for ATP1B3 antibody 67554-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Immunol

Integrative Transcriptomic, Proteomic and Functional Analysis Reveals ATP1B3 as a Diagnostic and Potential Therapeutic Target in Hepatocellular Carcinoma.

Authors - Shanshan Lu
  • KD Validated