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ATP1B3 Monoclonal antibody

ATP1B3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human and More (1)







Cat no : 67554-1-Ig


ATP1B3, ATPB 3, CD298, FLJ29027

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, human placenta tissue, THP-1 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67554-1-Ig targets ATP1B3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP1B3 fusion protein Ag30127
Full Name ATPase, Na+/K+ transporting, beta 3 polypeptide
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 38-43 kDa
GenBank Accession NumberBC011835
Gene Symbol ATP1B3
Gene ID (NCBI) 483
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).


Product Specific Protocols
WB protocol for ATP1B3 antibody 67554-1-IgDownload protocol
IHC protocol for ATP1B3 antibody 67554-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Immunol

Integrative Transcriptomic, Proteomic and Functional Analysis Reveals ATP1B3 as a Diagnostic and Potential Therapeutic Target in Hepatocellular Carcinoma.

Authors - Shanshan Lu
  • KD Validated