CoraLite® Plus 488-conjugated ATP1B3 Monoclonal antibody

ATP1B3 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H9C1

Cat no : CL488-67554

Synonyms

ATP1B3, ATPB 3, CD298, FLJ29027

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Validation Data Gallery

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  IF Staining of HEK-293 using CL488-67554

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 ATP1B3 antibody (CL488-67554, Clone: 1H9C1 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-67554

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ATP1B3 (CL488-67554, Clone:1H9C1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HEK-293 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67554 targets ATP1B3 in IF, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP1B3 fusion protein Ag30127
• Full Name: ATPase, Na+/K+ transporting, beta 3 polypeptide
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 38-43 kDa
• GenBank Accession Number: BC011835
• Gene Symbol: ATP1B3
• Gene ID (NCBI): 483
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).