ATP2A1 Polyclonal antibody

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  WB analysis using 22361-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22361-1-AP (ATP2A1 antibody) at dilution of 1:120000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 22361-1-AP

  IP result of anti-ATP2A1 (IP:22361-1-AP, 5ug; Detection:22361-1-AP 1:700) with mouse skeletal muscle tissue lysate 2160ug.

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  IHC staining of human skeletal muscle using 22361-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22361-1-AP (ATP2A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 22361-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22361-1-AP (ATP2A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse skeletal muscle using 22361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22361-1-AP (ATP2A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 22361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22361-1-AP (ATP2A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 22361-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22361-1-AP (ATP2A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 22361-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22361-1-AP (ATP2A1 Antibody) at dilution of 1:200 (under 40x lens).

ATP2A1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 22361-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

SR Ca(2+)-ATPase 1, SERCA1, EC:7.2.2.10, Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform, ATP2A 1

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue, human heart tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

22361-1-AP targets ATP2A1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP2A1 fusion protein Ag17944
• Full Name: ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
• Calculated Molecular Weight: 1001 aa, 110 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC037354
• Gene Symbol: ATP2A1
• Gene ID (NCBI): 487
• RRID: AB_2879089
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: O14983
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP2A1 also known as SERCA1, encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen and is involved in muscular excitation and contraction. Mutations in ATP2A1 cause some autosomal recessive forms of Brody disease(PMID: 23911890, 10914677).

Publications

Species	Application	Title
mouse	IHC,IF	Int J Mol Sci; The Effects of Aging on Sarcoplasmic Reticulum-Related Factors in the Skeletal Muscle of Mice; Authors - Yuji Kanazawa
mouse	WB	J Endocrinol; ChREBP-β regulates thermogenesis in brown adipose tissue.; Authors - Chunchun Wei
chicken	WB	Metallomics; Thioredoxin silencing-induced cardiac supercontraction occurs through endoplasmic reticulum stress and calcium overload in chicken.; Authors - Jie Yang
mouse	WB	Physiol Behav; Exposure to melamine cyanuric acid in adult mice caused motor activity and skeletal muscle energy metabolism disorder; Authors - Sijie Wang
mouse	WB	J Muscle Res Cell Motil; Differential regulation of Actn2 and Actn3 expression during unfolded protein response in C2C12 myotubes.; Authors - Nagakatsu Harada
	WB	Mol Ther Nucleic Acids; lnc-3215 Suppression Leads to Calcium Overload in Selenium Deficiency-Induced Chicken Heart Lesion via the lnc-3215-miR-1594-TNN2 Pathway.; Authors - Jie Yang