ATP2A1 Polyclonal antibody

ATP2A1 Polyclonal Antibody for IHC, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 22361-1-AP

Synonyms

ATP2A, ATP2A1, Calcium pump 1, SERCA1, SR Ca(2+) ATPase 1

---

Validation Data Gallery

• 

  IP experiment of mouse skeletal muscle using 22361-1-AP

  IP Result of anti-ATP2A1 (IP:22361-1-AP, 5ug; Detection:22361-1-AP 1:700) with mouse skeletal muscle tissue lysate 2160ug.

• 

  IHC staining of human skeletal muscle using 22361-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22361-1-AP (ATP2A1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22361-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22361-1-AP (ATP2A1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 22361-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22361-1-AP (ATP2A1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 22361-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22361-1-AP (ATP2A1 Antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 6 publications below

Product Information

The immunogen of 22361-1-AP is ATP2A1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP2A1 fusion protein Ag17944
• Full Name: ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
• Calculated molecular weight: 1001 aa, 110 kDa
• Observed molecular weight: 116 kDa
• GenBank accession number: BC037354
• Gene symbol: ATP2A1
• Gene ID (NCBI): 487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Endocrinol; ChREBP-β regulates thermogenesis in brown adipose tissue.; Authors - Chunchun Wei
chicken	WB	Metallomics; Thioredoxin silencing-induced cardiac supercontraction occurs through endoplasmic reticulum stress and calcium overload in chicken.; Authors - Jie Yang
mouse	WB	Physiol Behav; Exposure to melamine cyanuric acid in adult mice caused motor activity and skeletal muscle energy metabolism disorder; Authors - Sijie Wang
mouse	WB	J Muscle Res Cell Motil; Differential regulation of Actn2 and Actn3 expression during unfolded protein response in C2C12 myotubes.; Authors - Nagakatsu Harada
	WB	Mol Ther Nucleic Acids; lnc-3215 Suppression Leads to Calcium Overload in Selenium Deficiency-Induced Chicken Heart Lesion via the lnc-3215-miR-1594-TNN2 Pathway.; Authors - Jie Yang
human	WB	Acta Pharm Sin B; Highly expressed SERCA2 triggers tumor cell autophagy and is a druggable vulnerability in triple-negative breast cancer; Authors - Minmin Fan