ATP2A1 Polyclonal antibody

ATP2A1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22361-1-AP

Synonyms

ATP2A, ATP2A1, Calcium pump 1, serca, SERCA1, SR Ca(2+) ATPase 1



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

22361-1-AP targets ATP2A1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP2A1 fusion protein Ag17944
Full Name ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
Calculated molecular weight 1001 aa, 110 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC037354
Gene symbol ATP2A1
Gene ID (NCBI) 487
RRIDAB_2879089
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ATP2A1 antibody 22361-1-APDownload protocol
IHC protocol for ATP2A1 antibody 22361-1-APDownload protocol
IP protocol for ATP2A1 antibody 22361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Endocrinol

ChREBP-β regulates thermogenesis in brown adipose tissue.

Authors - Chunchun Wei
chickenWB

Metallomics

Thioredoxin silencing-induced cardiac supercontraction occurs through endoplasmic reticulum stress and calcium overload in chicken.

Authors - Jie Yang
mouseWB

Physiol Behav

Exposure to melamine cyanuric acid in adult mice caused motor activity and skeletal muscle energy metabolism disorder

Authors - Sijie Wang
mouseWB

J Muscle Res Cell Motil

Differential regulation of Actn2 and Actn3 expression during unfolded protein response in C2C12 myotubes.

Authors - Nagakatsu Harada
WB

Mol Ther Nucleic Acids

lnc-3215 Suppression Leads to Calcium Overload in Selenium Deficiency-Induced Chicken Heart Lesion via the lnc-3215-miR-1594-TNN2 Pathway.

Authors - Jie Yang
humanWB

Acta Pharm Sin B

Highly expressed SERCA2 triggers tumor cell autophagy and is a druggable vulnerability in triple-negative breast cancer

Authors - Minmin Fan