Validation Data Gallery
|Positive WB detected in
|human heart tissue, fetal human brain tissue
|Positive IHC detected in
|human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:100-1:400
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
60284-1-Ig targets ATP5C1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Mouse / IgG2a
|ATP5C1 fusion protein Ag1343
|ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
ATP5C1(ATP synthase subunit gamma, mitochondrial) is also named as ATP5C, ATP5CL1 and belongs to the ATPase gamma chain family. Some scientists reported the complete sequence of the gene for the human ATP synthase gamma subunit and described tissue-specific isoforms of the subunit generated by alternative splicing of exon 9. The liver (L) isoform differed from the heart (H) isoform by the addition of a single amino acid (asp273) at the C terminus. (PMID:8227057).
|Product Specific Protocols
|WB protocol for ATP5C1 antibody 60284-1-Ig
|IHC protocol for ATP5C1 antibody 60284-1-Ig
|IF protocol for ATP5C1 antibody 60284-1-Ig
|FC protocol for ATP5C1 antibody 60284-1-Ig
|Click here to view our Standard Protocols
Nucleic Acids Res
Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.
AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.
Nucleic Acids Res
Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.
Nat Cell Biol
Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space