ATP5C1 Monoclonal antibody

ATP5C1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1B5A2

Cat no : 60284-1-Ig

Synonyms

ATP5C, ATP5C1, ATP5CL1, F ATPase gamma subunit



Tested Applications

Positive WB detected inhuman heart tissue, fetal human brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

60284-1-Ig targets ATP5C1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, zebrafish
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATP5C1 fusion protein Ag1343
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC000931
Gene symbol ATP5C1
Gene ID (NCBI) 509
RRIDAB_2881402
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP5C1(ATP synthase subunit gamma, mitochondrial) is also named as ATP5C, ATP5CL1 and belongs to the ATPase gamma chain family. Some scientists reported the complete sequence of the gene for the human ATP synthase gamma subunit and described tissue-specific isoforms of the subunit generated by alternative splicing of exon 9. The liver (L) isoform differed from the heart (H) isoform by the addition of a single amino acid (asp273) at the C terminus. (PMID:8227057).

Protocols

Product Specific Protocols
WB protocol for ATP5C1 antibody 60284-1-IgDownload protocol
IHC protocol for ATP5C1 antibody 60284-1-IgDownload protocol
IF protocol for ATP5C1 antibody 60284-1-IgDownload protocol
FC protocol for ATP5C1 antibody 60284-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishWB

Nucleic Acids Res

Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.

Authors - Qinghai Zhang
humanWB

Autophagy

AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.

Authors - Zhenhong Ni
zebrafishWB

Nucleic Acids Res

Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.

Authors - Danni Chen
humanWB

Nat Cell Biol

Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space

Authors - Elias Adriaenssens