CoraLite®594-conjugated ATP5C1 Monoclonal antibody

ATP5C1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B5A2

Cat no : CL594-60284

Synonyms

ATP5C, ATP5C1, ATP5CL1, F ATPase gamma subunit

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-60284

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 ATP5C1 antibody (CL594-60284, Clone: 1B5A2 ) at dilution of 1:50.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60284 targets ATP5C1 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP5C1 fusion protein Ag1343
• Full Name: ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC000931
• Gene Symbol: ATP5C1
• Gene ID (NCBI): 509
• RRID: AB_2919912
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP5C1(ATP synthase subunit gamma, mitochondrial) is also named as ATP5C, ATP5CL1 and belongs to the ATPase gamma chain family. Some scientists reported the complete sequence of the gene for the human ATP synthase gamma subunit and described tissue-specific isoforms of the subunit generated by alternative splicing of exon 9. The liver (L) isoform differed from the heart (H) isoform by the addition of a single amino acid (asp273) at the C terminus. (PMID:8227057).