ATP5S Polyclonal antibody

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  WB analysis using 16335-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16335-1-AP (ATP5S antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

ATP5S Polyclonal Antibody for WB, ELISA

Cat No. 16335-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

FB, DMAC2L, Distal membrane arm assembly complex 2-like protein, ATPW, ATP synthase-coupling factor B

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Tested Applications

• Positive WB detected in: HL-60 cells, K-562 cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16335-1-AP targets ATP5S in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP5S fusion protein Ag9486
• Full Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit s (factor B)
• Calculated Molecular Weight: 127aa,15 kDa; 215aa,25 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC011549
• Gene Symbol: ATP5S
• Gene ID (NCBI): 27109
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99766
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DMAC2L also known as ATP5S, belongs to the ATP synthase family, which is a group of enzymes that play a crucial role in cellular energy production. ATP synthase is the central enzyme in oxidative phosphorylation, responsible for the production of most of the ATP in mammalian organisms. ATP5S is the S subunit (also known as factor B) of mitochondrial FO complex of ATP synthase. It is an essential component of the H+ channel of the FO complex (PMID:7706317, 6143319).

Publications

Species	Application	Title
human	WB	Biochem Genet; MiR-34a is Involved in the Decrease of ATP Contents Induced by Resistin Through Target on ATP5S in HepG2 Cells.; Authors - Fengyun Wen