ATP5S Polyclonal antibody

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• ATP5S Polyclonal antibody (16335-1-AP)

ATP5S Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16335-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ATP synthase-coupling factor B, ATPW, Distal membrane arm assembly complex 2-like protein, DMAC2L, FB

• 

  WB analysis using 16335-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16335-1-AP (ATP5S antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U2OS using 16335-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ATP5S antibody (16335-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HL-60 cells, K-562 cells, human placenta tissue
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16335-1-AP targets ATP5S in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9486

  Product name: Recombinant human ATP5S protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-127 aa of BC011549

  Sequence: MCCAVSEQRLTCADQMMLFGKISQQLCGVKKLPWSCDSRYFWGWLNAVFNKVDYDRIRDVGPDRAASEWLLRCGAMVRYHGQERWQKDYNHLPTGPLDKYKIQAIDATDSCIMSIGFDHMETSNICC

• Full Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit s (factor B)
• Calculated Molecular Weight: 127aa,15 kDa; 215aa,25 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC011549
• Gene Symbol: ATP5S
• Gene ID (NCBI): 27109
• RRID: AB_3741884
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99766
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DMAC2L also known as ATP5S, belongs to the ATP synthase family, which is a group of enzymes that play a crucial role in cellular energy production. ATP synthase is the central enzyme in oxidative phosphorylation, responsible for the production of most of the ATP in mammalian organisms. ATP5S is the S subunit (also known as factor B) of mitochondrial FO complex of ATP synthase. It is an essential component of the H+ channel of the FO complex (PMID:7706317, 6143319).

Publications

What published studies show

The ATP5S Polyclonal antibody (Cat# 16335-1-AP) has 1 citation. It appears in Biochemical Genetics (2015), in a study investigating how miR-34a mediates resistin-induced ATP depletion by targeting ATP5S in HepG2 hepatocellular carcinoma cells. The associated research fields include microRNA regulation, cellular energy metabolism (ATP homeostasis), and liver cancer biology. The antibody was validated by Western blot in human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Biochem Genet; MiR-34a is Involved in the Decrease of ATP Contents Induced by Resistin Through Target on ATP5S in HepG2 Cells.; Authors - Fengyun Wen