ATP6V1B1 Monoclonal antibody

ATP6V1B1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G10A8

Cat no : 68219-1-Ig

Synonyms

ATP6B1, ATP6V1B1, RTA1B, V ATPase subunit B 1, VATB, VMA2, VPP3

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Validation Data Gallery

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  WB analysis using 68219-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68219-1-Ig (ATP6V1B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 68219-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP6V1B1 antibody (68219-1-Ig, Clone: 1G10A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: JAR cells, pig brain tissue, pig cerebellum tissue, rabbit brain tissue, rabbit cerebellum tissue, rat kidney tissue, rat brain tissue, mouse kidney tissue, mouse brain tissue
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68219-1-Ig targets ATP6V1B1 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP6V1B1 fusion protein Ag7405
• Full Name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC063411
• Gene Symbol: ATP6V1B1
• Gene ID (NCBI): 525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.