Tested Applications
| Positive WB detected in | JAR cells, pig brain tissue, pig cerebellum tissue, rabbit brain tissue, rabbit cerebellum tissue, rat kidney tissue, rat brain tissue, mouse kidney tissue, mouse brain tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68219-1-Ig targets ATP6V1B1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | c.elegans |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7405 Product name: Recombinant human ATP6V1B1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-327 aa of BC063411 Sequence: MAMEIDSRPGGLPGSSCNLGAAREHMQAVTRNYITHPRVTYRTVCSVNGPLVVLDRVKFAQYAEIVHFTLPDGTQRSGQVLEVAGTKAIVQVFEGTSGIDARKTTCEFTGDILRTPVSEDMLGRVFNGSGKPIDKGPVVMAEDFLDINGQPINPHSRIYPEEMIQTGISPIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKAVLDYHDDNFAIVFAAMGVNMETARFFKSDFEQNGTMGNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLAT Predict reactive species |
| Full Name | ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC063411 |
| Gene Symbol | ATP6V1B1 |
| Gene ID (NCBI) | 525 |
| RRID | AB_2935307 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P15313 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP6V1B1 antibody 68219-1-Ig | Download protocol |
| WB protocol for ATP6V1B1 antibody 68219-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATP6V1B1 Monoclonal antibody (1G10A8), catalog number 68219-1-Ig, has 1 total citation. The citation appears in the Journal of Biological Chemistry (J Biol Chem), published in 2025. The associated research field involves lipid metabolism and lysosomal biology in Caenorhabditis elegans, specifically examining how FAT-5/SCD5-mediated lipid localization in lysosomes alleviates gamma radiation injury. The antibody was used for Western blot (WB) in this study.
| Species | Application | Title |
|---|---|---|
J Biol Chem FAT-5/SCD5-mediated lipid localization in lysosomes alleviates gamma radiation injury in Caenorhabditis elegans. |
