ATP6V1D Polyclonal antibody

ATP6V1D Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 14920-1-AP

Synonyms

ATP6M, ATP6V1D, V ATPase subunit D, V type proton ATPase subunit D, Vacuolar proton pump subunit D, VATD, VMA8

---

Validation Data Gallery

• 

  WB analysis of human brain using 14920-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14920-1-AP (ATP6V1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14920-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14920-1-AP (ATP6V1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 14920-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14920-1-AP (ATP6V1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14920-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14920-1-AP (ATP6V1D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 14920-1-AP

  IP result of anti-ATP6V1D (IP:14920-1-AP, 4ug; Detection:14920-1-AP 1:500) with mouse lung tissue lysate 4000ug.

• 

  IHC staining of human lung cancer using 14920-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14920-1-AP (ATP6V1D antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 14920-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14920-1-AP (ATP6V1D antibody) at dilution of 1:100 (under 10x lens).

---

Tested Applications

• Positive WB detected in: human brain tissue, mouse skeletal muscle tissue, mouse lung tissue, rat lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IP: See 1 publications below

Product Information

14920-1-AP targets ATP6V1D in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP6V1D fusion protein Ag6737
• Full Name: ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC001411
• Gene Symbol: ATP6V1D
• Gene ID (NCBI): 51382
• RRID: AB_2243302
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP6V1D is also named as ATP6M, VATD(V-type proton ATPase subunit D) and belongs to the V-ATPase D subunit family. ATP6V1D gene has been under strong negative selection during evolution and is highly conserved among mammals, flies, worms, yeast, plants, and bacteria(PMID:11435709). It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.

Publications

Species	Application	Title
mouse	WB	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio
mouse	WB	J Ethnopharmacol; Tangshen formula protects against podocyte apoptosis via enhancing the TFEB-mediated autophagy-lysosome pathway in diabetic nephropathy; Authors - Yuyang Wang
rat	WB	J Proteome Res; Quantitative proteome of medulla oblongata in spontaneously hypertensive rats.; Authors - Sun Dongmei D
human	WB	Mol Cell Proteomics; Multi Cell Line Analysis of Lysosomal Proteomes Reveals Unique Features and Novel Lysosomal Proteins; Authors - Fatema Akter
human	WB	Autophagy; Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate; Authors - Mengting Ou
human	WB	Nat Commun; Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo; Authors - Jasjot Singh