ATX3,ATXN3 Polyclonal antibody

• 

  WB analysis using 13505-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13505-1-AP

  HEK-293 (WT and ATXN3 KO) lysates prepared with RIPA buffer, 30 μg protein loaded. 13505-1-AP incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  WB analysis of HEK-293 using 13505-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13505-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 13505-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 13505-1-AP

  HEK-293 lysates prepared and IP of ATXN3 performed using 1.0 μg of 13505-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  IHC staining of human pancreas cancer using 13505-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 13505-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:100 (under 10x lens).

• Featured Product
• KD/KO Validated

ATX3,ATXN3 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 13505-1-AP

Publications(26)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

AT3, ataxin 3, ATX3, ATX3,ATXN3, ATXN3, JOS, MJD, MJD1, SCA3

---

Tested Applications

• Positive WB detected in: Neuro-2a cells, mouse heart tissue, mouse placenta tissue, HEK-293 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 23 publications below
• IHC: See 4 publications below
• IF: See 6 publications below
• IP: See 3 publications below
• CoIP: See 3 publications below

Product Information

13505-1-AP targets ATX3,ATXN3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig, caenorhabditis elegans
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4341

  Product name: Recombinant human ATX3,ATXN3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-370 aa of BC033711

  Sequence: MESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDLPDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGSGMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMTQTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQQQQQQQQQQGDLSGQSSHPCERPATSSGALGSDLGDAMSEEDMLQAAVTMSLETVRNDLKTEGKK

• Full Name: ataxin 3
• Calculated Molecular Weight: 370 aa, 43 kDa
• Observed Molecular Weight: 35-42 kDa
• GenBank Accession Number: BC033711
• Gene Symbol: ATXN3
• Gene ID (NCBI): 4287
• RRID: AB_2061192
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P54252
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
human	WB	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu ; KD Validated
human	WB, IF, IP	PLoS Genet; The Role of the Mammalian DNA End-processing Enzyme Polynucleotide Kinase 3'-Phosphatase in Spinocerebellar Ataxia Type 3 Pathogenesis.; Authors - Arpita Chatterjee ; KD Validated
human	WB	Sci Total Environ; USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.; Authors - Lei Zhang
human	WB	PLoS Genet; Inactivation of PNKP by mutant ATXN3 triggers apoptosis by activating the DNA damage-response pathway in SCA3.; Authors - Rui Gao
mouse	WB	Neurobiol Dis; A new humanized ataxin-3 knock-in mouse model combines the genetic features, pathogenesis of neurons and glia and late disease onset of SCA3/MJD.; Authors - Pawel M Switonski