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- KD/KO Validated
ATX3,ATXN3 Polyclonal antibody
ATX3,ATXN3 Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 13505-1-AP
Validation Data Gallery
|Positive WB detected in||Neuro-2a cells, mouse heart tissue, mouse placenta tissue, HEK-293 cells, mouse brain tissue, rat brain tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13505-1-AP targets ATX3,ATXN3 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig, Caenorhabditis elegans|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATX3,ATXN3 fusion protein Ag4341|
|Full Name||ataxin 3|
|Calculated molecular weight||370 aa, 43 kDa|
|Observed molecular weight||35-42 kDa|
|GenBank accession number||BC033711|
|Gene ID (NCBI)||4287|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.
An Interaction Landscape of Ubiquitin Signaling.
TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.
The Role of the Mammalian DNA End-processing Enzyme Polynucleotide Kinase 3'-Phosphatase in Spinocerebellar Ataxia Type 3 Pathogenesis.
Sci Total Environ
USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.
Inactivation of PNKP by mutant ATXN3 triggers apoptosis by activating the DNA damage-response pathway in SCA3.
A new humanized ataxin-3 knock-in mouse model combines the genetic features, pathogenesis of neurons and glia and late disease onset of SCA3/MJD.