Ace2 Polyclonal antibody

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  WB analysis using 32628-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32628-1-AP (Ace2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 32628-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 32628-1-AP (Ace2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 32628-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 32628-1-AP (Ace2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Ace2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32628-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ACE-related carboxypeptidase, Angiotensin-converting enzyme 2, EC:3.4.17.-, EC:3.4.17.23, Processed angiotensin-converting enzyme 2

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse small intestine tissue, rat small intestine tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32628-1-AP targets Ace2 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ace2 fusion protein Eg2834
• Full Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• Calculated Molecular Weight: 92 kDa
• Observed Molecular Weight: 110 kDa, 120 kDa
• GenBank Accession Number: NM_001130513.1
• Gene Symbol: Ace2
• Gene ID (NCBI): 70008
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8R0I0-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACE2 (Angiotensin-Converting Enzyme 2) is a key regulator of the renin-angiotensin system (RAS) and plays a crucial role in maintaining cardiovascular homeostasis. It is also the functional receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for COVID-19.