Albumin Monoclonal antibody, PBS Only (Detector)

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  WB analysis of human plasma using 66051-1-Ig (same clone as 66051-1-PBS)

  human plasma (diluted 5000 fold) was subjected to SDS PAGE followed by western blot with 66051-1-Ig (Albumin Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 66051-1-Ig (ALB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50031-1

  Sandwich ELISA standard curve of MP50031-1, Albumin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66051-2-PBS. Detection antibody: 66051-1-PBS. Standard: Ag9885. Range: 50-3200 ng/mL.

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  Sample test of MP50031-1

  Human cerebrospinal fluid (CSF) of one individual human donors was measured. The Albumin concentration of detected samples was determined to be 29.50 μg/mL.

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  Sample test of MP50031-1

  Serum of four individual healthy human donors was measured. The Albumin concentration of detected samples was determined to be 29.65 mg/mL with a range of 11.40-48.00 mg/mL.

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  Sample test of MP50031-1

  Urine of four individual healthy human donors was measured. The Albumin concentration of detected samples was determined to be 1,036.00 ng/mL with a range of 560.00-1,616.00 ng/mL.

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  Sample test of MP50031-1

  Saliva of two individual healthy human donors was measured. The Albumin concentrations of detected samples were determined to be 32.00 μg/mL and 18.60 μg/mL, respectively.

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  Sample test of MP50031-1

  Human milk of two individual healthy human donors was measured. The Albumin concentrations of detected samples were determined to be 106.00 μg/mL and 116.00 μg/mL, respectively.

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  IP experiment of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

  IP result of anti-Albumin (IP:66051-1-Ig, 4ug; Detection:66051-1-Ig 1:1000) with HepG2 cells lysate 1720 ug. This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66051-1-Ig (Albumin antibody) at dilution of 1:64000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 66051-1-Ig (same clone as 66051-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 66051-1-Ig (same clone as 66051-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 66051-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 66051-1-Ig against Human Albumin were performed. The affinity constant is 0.574 nM

Albumin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 66051-1-PBS

Clone No.4A1C11

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

ALB, 4A1C11, HSA, Serum albumin

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Product Information

66051-1-PBS targets Albumin as part of a matched antibody pair:

MP50031-1: 66051-2-PBS capture and 66051-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9885

  Product name: Recombinant human Albumin protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 256-609 aa of BC034023

  Sequence: SKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL

• Full Name: albumin
• Calculated Molecular Weight: 609 aa, 69 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC034023
• Gene Symbol: Albumin
• Gene ID (NCBI): 213
• RRID: AB_11042320
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P02768
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071) In additon, glycated serum albumin is also a potential diabetes biomarker.