Albumin Antibody

Albumin Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4A1C11

Cat no : 66051-1-Ig

Synonyms

Alb, albumin, Serum albumin



Tested Applications

Positive WB detected inhuman plasma tissue, human liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 66051-1-Ig immunogen is Fusion Protein Albumin protein expressed in E. coli.

Reactivity human, rat, pig
Cited Species human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Albumin fusion protein Ag9885
Full Name albumin
Calculated molecular weight 609aa,69 kDa
Observed molecular weight 66 kDa
GenBank accession number BC034023
Gene symbol ALB
Gene ID (NCBI) 213
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)

Protocols

Product Specific Protocols
WB protocol for Albumin antibody 66051-1-IgDownload protocol
IHC protocol for Albumin antibody 66051-1-IgDownload protocol
IF protocol for Albumin antibody 66051-1-IgDownload protocol
IP protocol for Albumin antibody 66051-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Neurosci Methods

Total Protein is an Effective Loading Control for Cerebrospinal Fluid Western Blots.

Authors - Mahlon A Collins
ratIF

Stem Cell Res Ther

Treatment with adipose tissue-derived mesenchymal stem cells exerts anti-diabetic effects, improves long-term complications, and attenuates inflammation in type 2 diabetic rats.

Authors - Songyan Yu

Adv Healthc Mater

Inhibition of Kupffer Cell Autophagy Abrogates Nanoparticle-Induced Liver Injury.

Authors - Shasha Zhu
humanWB

Small

Persistency of Enlarged Autolysosomes Underscores Nanoparticle-Induced Autophagy in Hepatocytes.

Authors - Ji-Qian Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yuan Lyu (10-15-2019)

This Albumin antibody is working very well on human seminal plasma and blood plasma.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Human seminal plasma and blood plasma
No image submitted with review