Albumin Monoclonal antibody
Albumin Monoclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, pig
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
4A1C11
Cat no : 66051-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma tissue, human liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
The immunogen of 66051-1-Ig is Albumin Fusion Protein expressed in E. coli.
| Tested Reactivity | human, rat, pig |
| Cited Reactivity | human, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Albumin fusion protein Ag9885 |
| Full Name | albumin |
| Calculated molecular weight | 609 aa, 69 kDa |
| Observed molecular weight | 66 kDa |
| GenBank accession number | BC034023 |
| Gene symbol | ALB |
| Gene ID (NCBI) | 213 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Albumin antibody 66051-1-Ig | Download protocol |
| IHC protocol for Albumin antibody 66051-1-Ig | Download protocol |
| IF protocol for Albumin antibody 66051-1-Ig | Download protocol |
| IP protocol for Albumin antibody 66051-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Neurosci Methods Total Protein is an Effective Loading Control for Cerebrospinal Fluid Western Blots. | ||
Stem Cell Res Ther Treatment with adipose tissue-derived mesenchymal stem cells exerts anti-diabetic effects, improves long-term complications, and attenuates inflammation in type 2 diabetic rats. | ||
FASEB J Anti-human serum albumin autoantibody may be involved in the pathogenesis of autoimmune bullous skin diseases. | ||
Adv Healthc Mater Inhibition of Kupffer Cell Autophagy Abrogates Nanoparticle-Induced Liver Injury. | ||
Small Persistency of Enlarged Autolysosomes Underscores Nanoparticle-Induced Autophagy in Hepatocytes. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Yuan (Verified Customer) (10-15-2019) | This Albumin antibody is working very well on human seminal plasma and blood plasma.
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