Albumin Monoclonal antibody

Albumin Monoclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig and More (2)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

4A1C11

Cat no : 66051-1-Ig

Synonyms

Alb, albumin, Serum albumin


"Albumin Antibodies" Comparison

View side-by-side comparison of Albumin antibodies from other vendors to find the one that best suits your research needs.
  • IHC staining of human liver using 66051-1-Ig

    IHC staining of human liver using 66051-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 66051-1-Ig

    IHC staining of human liver using 66051-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of human plasma using 66051-1-Ig

    WB analysis of human plasma using 66051-1-Ig

    human plasma (diluted 5000 fold) was subjected to SDS PAGE followed by western blot with 66051-1-Ig (Albumin Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 66051-1-Ig

    IP experiment of HepG2 using 66051-1-Ig

    IP Result of anti-Albumin (IP:66051-1-Ig, 5ug; Detection:66051-1-Ig 1:5000) with HepG2 cells lysate 1800ug.

  • IF Staining of HepG2 using 66051-1-Ig

    IF Staining of HepG2 using 66051-1-Ig

    Immunofluorescent analysis of HepG2 cells using 66051-1-Ig (Albumin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).


Tested Applications

Positive WB detected inhuman plasma tissue, human liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66051-1-Ig is Albumin Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, pig
Cited Reactivity human, mouse, rabbit, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Albumin fusion protein Ag9885
Full Name albumin
Calculated molecular weight 609 aa, 69 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC034023
Gene symbol ALB
Gene ID (NCBI) 213
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071) In additon, glycated serum albumin is also a potential diabetes biomarker.

Protocols

Product Specific Protocols
WB protocol for Albumin antibody 66051-1-IgDownload protocol
IHC protocol for Albumin antibody 66051-1-IgDownload protocol
IF protocol for Albumin antibody 66051-1-IgDownload protocol
IP protocol for Albumin antibody 66051-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Neurosci Methods

Total Protein is an Effective Loading Control for Cerebrospinal Fluid Western Blots.

Authors - Mahlon A Collins
humanIF

Cell Death Dis

WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1.

Authors - Zhe Zheng
mouseIF

Nanotechnology

Kupffer Cell-targeting strategy for the protection of Hepatic Ischemia/Reperfusion Injury.

Authors - Qingqing Dai
rabbitWB

Life Sci

Continuous zebularine treatment enhances hepatic differentiation of mesenchymal stem cells under liver-specific factors induction in vitro.

Authors - Juan Chen
ratIF

Stem Cell Res Ther

Treatment with adipose tissue-derived mesenchymal stem cells exerts anti-diabetic effects, improves long-term complications, and attenuates inflammation in type 2 diabetic rats.

Authors - Songyan Yu
humanIP,WB

FASEB J

Anti-human serum albumin autoantibody may be involved in the pathogenesis of autoimmune bullous skin diseases.

Authors - Hua Qian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yuan (Verified Customer) (10-15-2019)

This Albumin antibody is working very well on human seminal plasma and blood plasma.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Human seminal plasma and blood plasma