Albumin Monoclonal antibody

Albumin Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4A1C11

Cat no : 66051-1-Ig

Synonyms

Alb, albumin, HSA, Serum albumin


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Applications:
  • Western Blot (WB) analysis of human plasma using Albumin Monoclonal antibody (66051-1-Ig)

    WB analysis of human plasma using 66051-1-Ig

    human plasma (diluted 5000 fold) was subjected to SDS PAGE followed by western blot with 66051-1-Ig (Albumin Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using Albumin Monoclonal antibody (66051-1-Ig)

    WB analysis of human liver using 66051-1-Ig

    human liver tissue were subjected to SDS PAGE followed by western blot with 66051-1-Ig (ALB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using Albumin Monoclonal antibody (66051-1-Ig)

    IP experiment of HepG2 using 66051-1-Ig

    IP Result of anti-Albumin (IP:66051-1-Ig, 5ug; Detection:66051-1-Ig 1:5000) with HepG2 cells lysate 1800ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using Albumin Monoclonal antibody (66051-1-Ig)

    IHC staining of human liver using 66051-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Albumin Monoclonal antibody (66051-1-Ig)

    IHC staining of human liver using 66051-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Albumin Monoclonal antibody (66051-1-Ig)

    IF Staining of HepG2 using 66051-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Albumin Monoclonal antibody (66051-1-Ig)

    IF Staining of HepG2 using 66051-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).


Tested Applications

Positive WB detected inhuman plasma tissue, human liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66051-1-Ig is Albumin Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, pig
Cited Reactivityhuman, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Albumin fusion protein Ag9885
Full Name albumin
Calculated molecular weight 609 aa, 69 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC034023
Gene symbol ALB
Gene ID (NCBI) 213
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071) In additon, glycated serum albumin is also a potential diabetes biomarker.

Protocols

Product Specific Protocols
WB protocol for Albumin antibody 66051-1-IgDownload protocol
IHC protocol for Albumin antibody 66051-1-IgDownload protocol
IF protocol for Albumin antibody 66051-1-IgDownload protocol
IP protocol for Albumin antibody 66051-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
mouseWB

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang
humanIF

J Hepatol

Targeting NFATc4 attenuates non-alcoholic steatohepatitis in mice.

Authors - Meng Du
humanWB

J Extracell Vesicles

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

Authors - Xiaohan Gao
humanWB

Small

Persistency of Enlarged Autolysosomes Underscores Nanoparticle-Induced Autophagy in Hepatocytes.

Authors - Ji-Qian Zhang
humanIF

Biofabrication

Dual-core coaxial bioprinting of double-channel constructs with a potential for perfusion and interaction of cells.

Authors - Yanrong Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yuan (Verified Customer) (10-15-2019)

This Albumin antibody is working very well on human seminal plasma and blood plasma.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Human seminal plasma and blood plasma