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Albumin Antibody 3 Publications

Mouse Monoclonal| Catalog number: 66051-1-Ig |CloneNo.: 4A1C11

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Con: 107 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Albumin Antibody IHC human liver tissue 66051-1-Ig

Immunohistochemical of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 10x lens)

Albumin Antibody IHC human liver tissue 66051-1-Ig

Immunohistochemical of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 40x lens)

Albumin Antibody WB human plasma tissue 66051-1-Ig

human plasma (diluted 5000 fold) was subjected to SDS PAGE followed by western blot with 66051-1-Ig (Albumin Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours

Albumin Antibody IP HepG2 cells 66051-1-Ig

IP Result of anti-Albumin (IP:66051-1-Ig, 5ug; Detection:66051-1-Ig 1:5000) with HepG2 cells lysate 1800ug.

Albumin Antibody IF HepG2 cells 66051-1-Ig

Immunofluorescent analysis of HepG2 cells using 66051-1-Ig(Albumin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, rat, pig
Positive WB detected in:
human plasma tissue, human liver tissue
Positive IP detected in:
HepG2 cells
Positive IHC detected in:
human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Albumin fusion protein Ag9885
Full name:
albumin
Calculated molecular weight:
609aa,69 kDa
Observed molecular weight:
66 kDa
GenBank accession number:
BC034023
Gene ID (NCBI):
213
Gene symbol
ALB
Synonyms
Alb, albumin, Serum albumin

Background

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)


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