B3GNT3 Recombinant monoclonal antibody

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  WB analysis using 85713-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85713-1-RR (B3GNT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity of 85713-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85637-2-RR against Human B3GNT3 were performed. The affinity constant is 3.39 nM.

B3GNT3 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85713-1-RR

Clone No.242979D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

B3GALT8, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase, Beta-1,3-galactosyltransferase 8, Beta-1,3-GalTase 8, Beta-1,3-Gn-T3

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Tested Applications

• Positive WB detected in: HeLa cells, NCI-H1299 cells, SH-SY5Y cells, SW 1990 cells, A549 cells, HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85713-1-RR targets B3GNT3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag12061

  Product name: Recombinant human B3GNT3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 34-372 aa of BC066876

  Sequence: CKVQEQPPAIPEALAWPTPPTRPAPAPCHANTSMVTHPDFATQPQHVQNFLLYRHYRHFPLLQDVPPSKCAQPVFLLLVIKSSPSNYVRRELLRRTWGRERKVRGLQLRLLFLVGTASNPHEARKVNRLLELEAQTHGDILQWDFHDSFFNLTLKQVLFLQWQETRCANASFVLNGDDDVFAHTDNMVFYLQDHDPGRHLFVGQLIQNVGPIRAFWSKYYVPEVVTQNERYPPYCGGGGFLLSRFTAAALRRAAHVLDIFPIDDVFLGMCLELEGLKPASHSGIRTSGVRAPSQHLSSFDPCFYRDLLLVHRFLPYEMLLMWDALNQPNLTCGNQTQIY

• Full Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
• Calculated Molecular Weight: 372 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC066876
• Gene Symbol: B3GNT3
• Gene ID (NCBI): 10331
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y2A9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-1,3-N-acetylglucosaminyltransferase 3 (B3GNT3) is a type II transmembrane protein located in the Golgi apparatus and contributes to the regulation of L-selectin ligand function, lymphocyte trafficking, and T cell homing. B3GNT3 is overexpressed in breast cancer, and also plays a critical role in the aggressiveness of pancreatic cancer (PMID: 33718036, 30466404). Western blot analysis detected B3GNT3 at an apparent molecular mass of 43 kDa.