B4GALNT2 Polyclonal antibody

B4GALNT2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 24024-1-AP

Synonyms

B4GALGT2, B4GALNT2, B4GALT, Cad, GALGT2, SD, Sda

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Validation Data Gallery

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  WB analysis of Jurkat using 24024-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 24024-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 24024-1-AP (B4GALNT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 24024-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 24024-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 24024-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 24024-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, COLO 320 cells
• Positive IHC detected in: human colon cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24024-1-AP is B4GALNT2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B4GALNT2 fusion protein Ag21166
• Full Name: beta-1,4-N-acetyl-galactosaminyl transferase 2
• Calculated molecular weight: 566 aa, 63 kDa
• Observed molecular weight: 65 kDa
• GenBank accession number: BC113677
• Gene symbol: B4GALNT2
• Gene ID (NCBI): 124872
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B4GALNT2, also named as GALGT2, catalyzes the last step in the biosynthesis of the human Sd(a) antigen, which is found on more than 90% of Caucasian red blood cells. It also catalyzes the last step in the biosynthesis of the Cad antigen, which is related both serologically and biochemically to the Sd(a) antigen. B4GALNT2 transfers a beta-1,4-linked GalNAc to the galactose residue of an alpha-2,3-sialylated chain. This antibody detects the 55-65 kDa B4GALNT2 protein.