BACE2 Polyclonal antibody, PBS Only

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  WB analysis using 16321-1-AP (same clone as 16321-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16321-1-AP (BACE2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16321-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 16321-1-AP (same clone as 16321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16321-1-AP (BACE2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16321-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 16321-1-AP (same clone as 16321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16321-1-AP (BACE2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16321-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 16321-1-AP (same clone as 16321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16321-1-AP (BACE2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16321-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 16321-1-AP (same clone as 16321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16321-1-AP (BACE2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16321-1-PBS in a different storage buffer formulation.

BACE2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 16321-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

16321-1-PBS targets BACE2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9404

  Product name: Recombinant human BACE2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 39-398 aa of BC014453

  Sequence: TNRVVAPTPGPGTPAERHADGLALALEPALASPAGAANFLAMVDNLQGDSGRGYYLEMLIGTPPQKLQILVDTGSSNFAVAGTPHSYIDTYFDTERSSTYRSKGFDVTVKYTQGSWTGFVGEDLVTIPKGFNTSFLVNIATIFESENFFLPGIKWNGILGLAYATLAKPSSSLETFFDSLVTQANIPNVFSMQMCGAGLPVAGSGTNGGSLVLGGIEPSLYKGDIWYTPIKEEWYYQIEILKLEIGGQSLNLDCREYNADKAIVDSGTTLLRLPQKVFDAVVEAVARASLIPEFSDGFWTGSQLACWTNSETPWSYFPKISIYLRDENSSRSFRITILPQLYIQPMMGAGLNYECYRFGI

• Full Name: beta-site APP-cleaving enzyme 2
• Calculated Molecular Weight: 518 aa, 56 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC014453
• Gene Symbol: BACE2
• Gene ID (NCBI): 25825
• RRID: AB_2061667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5Z0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.