BAD Polyclonal antibody
BAD Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human and More (2)
WB, IHC, FC, ELISA
Cat no : 10435-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, Jurkat cells, HepG2 cells, Neuro-2a cells|
|Positive IHC detected in||human prostate hyperplasia tissue, human prostate cancer tissue, mouse liver tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:500|
|Sample-dependent, check data in validation data gallery|
10435-1-AP targets BAD in WB, IHC, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BAD fusion protein Ag0688|
|Full Name||BCL2-associated agonist of cell death|
|Calculated molecular weight||18 kDa|
|Observed molecular weight||18-25 kDa|
|GenBank accession number||BC001901|
|Gene ID (NCBI)||572|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
|Product Specific Protocols|
|WB protocol for BAD antibody 10435-1-AP||Download protocol|
|IHC protocol for BAD antibody 10435-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Carly (Verified Customer) (11-17-2020)
Tested using EDTA plasma on an antibody microarray