Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, HepG2 cells, Neuro-2a cells |
| Positive IHC detected in | human prostate hyperplasia tissue, human prostate cancer tissue, mouse liver tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 105 publications below |
| IHC | See 5 publications below |
Product Information
10435-1-AP targets BAD in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, canine, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0688 Product name: Recombinant human BAD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 14-168 aa of BC001901 Sequence: DSSSAERGLGPSPAGDGPSGSGKHHRQAPGLLWDASHQQEQPTSSSHHGGAGAVEIRSRHSSYPAGTEDDEGMGEEPSPFRGRSRSAPPNLWAAQRYGRELRRMSDEFVDSFKKGLPRPKSAGTATQMRQSSSWTRVFQSWWDRNLGRGSSAPSQ Predict reactive species |
| Full Name | BCL2-associated agonist of cell death |
| Calculated Molecular Weight | 18 kDa |
| Observed Molecular Weight | 18-25 kDa |
| GenBank Accession Number | BC001901 |
| Gene Symbol | BAD |
| Gene ID (NCBI) | 572 |
| RRID | AB_2061994 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92934 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BAD antibody 10435-1-AP | Download protocol |
| WB protocol for BAD antibody 10435-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Carbohydr Polym A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer | ||
Oxid Med Cell Longev Cystathionine β-Synthase Regulates the Proliferation, Migration, and Invasion of Thyroid Carcinoma Cells. | ||
Oxid Med Cell Longev Dihydroartemisinin Modulates Apoptosis and Autophagy in Multiple Myeloma through the P38/MAPK and Wnt/β-Catenin Signaling Pathways. | ||
Int J Nanomedicine Synergism of cisplatin-oleanolic acid co-loaded calcium carbonate nanoparticles on hepatocellular carcinoma cells for enhanced apoptosis and reduced hepatotoxicity. | ||
Cell Death Dis CRB3 regulates contact inhibition by activating the Hippo pathway in mammary epithelial cells. | ||
Int J Oncol CUDC‑101 is a potential target inhibitor for the EGFR‑overexpression bladder cancer cells |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Carly (Verified Customer) (11-17-2020) | Tested using EDTA plasma on an antibody microarray
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