|Positive WB detected in||Jurkat cells, Tunicamycin treated HeLa cells|
|Positive IHC detected in||human prostate hyperplasia tissue, human lymphoma tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:500|
|Sample-dependent, check data in validation data gallery|
10435-1-AP targets BAD in WB, IHC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BAD fusion protein Ag0688|
|Full Name||BCL2-associated agonist of cell death|
|Calculated molecular weight||18 kDa|
|Observed molecular weight||18-25 kDa|
|GenBank accession number||BC001901|
|Gene ID (NCBI)||572|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
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