BAD Polyclonal antibody
BAD Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, FC, ELISA
Conjugate
Unconjugated
Cat no : 10435-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, HepG2 cells, Neuro-2a cells |
| Positive IHC detected in | human prostate hyperplasia tissue, human prostate cancer tissue, mouse liver tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 77 publications below |
| IHC | See 2 publications below |
Product Information
10435-1-AP targets BAD in WB, IHC, FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BAD fusion protein Ag0688 |
| Full Name | BCL2-associated agonist of cell death |
| Calculated molecular weight | 18 kDa |
| Observed molecular weight | 18-25 kDa |
| GenBank accession number | BC001901 |
| Gene symbol | BAD |
| Gene ID (NCBI) | 572 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BAD antibody 10435-1-AP | Download protocol |
| IHC protocol for BAD antibody 10435-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Carbohydr Polym A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer | ||
Cell Death Dis CRB3 regulates contact inhibition by activating the Hippo pathway in mammary epithelial cells. | ||
Oncotarget Targeting ERK enhances the cytotoxic effect of the novel PI3K and mTOR dual inhibitor VS-5584 in preclinical models of pancreatic cancer. | ||
Biochem Pharmacol Chlorpromazine, an antipsychotic agent, induces G2/M phase arrest and apoptosis via regulation of the PI3K/AKT/mTOR-mediated autophagy pathways in human oral cancer. | ||
Oxid Med Cell Longev Hydrogen Sulfide Alleviates Lipopolysaccharide-Induced Diaphragm Dysfunction in Rats by Reducing Apoptosis and Inflammation through ROS/MAPK and TLR4/NF-κB Signaling Pathways. | ||
Ecotoxicol Environ Saf Prenatal exposure to di-n-butyl phthalate induces erectile dysfunction in male adult rats. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Carly (Verified Customer) (11-17-2020) | Tested using EDTA plasma on an antibody microarray
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