|Positive WB detected in||mouse brain tissue, rat brain tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
27215-1-AP targets BAF53B in WB, ELISA applications and shows reactivity with mouse, rat samples.
|Tested Reactivity||mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BAF53B fusion protein Ag25745|
|Full Name||actin-like 6B|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||47-53 kDa|
|GenBank accession number||BC020944|
|Gene ID (NCBI)||51412|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
BAF53B, also named as ACTL6B, encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. BAF53b is unique among nucleosome remodeling complex subunits because it is neuron specific and is not found in any other nucleosome remodeling complex besides the nBAF complex(PMID: 23525042). BAF53B can be detected as about 47-53 kDa.