BAF53B Polyclonal antibody

BAF53B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27215-1-AP

Synonyms

actin like 6B, Actin like protein 6B, Actin related protein Baf53b, ACTL6, ACTL6B, ArpNalpha, BAF53B, BRG1 associated factor 53B

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Validation Data Gallery

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  WB analysis of mouse brain using 27215-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27215-1-AP (BAF53B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 27215-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 27215-1-AP (BAF53B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

27215-1-AP targets BAF53B in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BAF53B fusion protein Ag25745
• Full Name: actin-like 6B
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47-53 kDa
• GenBank Accession Number: BC020944
• Gene Symbol: BAF53B
• Gene ID (NCBI): 51412
• RRID: AB_2857952
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAF53B, also named as ACTL6B, encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. BAF53b is unique among nucleosome remodeling complex subunits because it is neuron specific and is not found in any other nucleosome remodeling complex besides the nBAF complex(PMID: 23525042). BAF53B can be detected as about 47-53 kDa.

Publications

Species	Application	Title
human	WB	J Neurosci Res; An epilepsy-associated ACTL6B variant captures neuronal hyperexcitability in a human induced pluripotent stem cell model.; Authors - Lucie Y Ahn