BAG2 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 85063-4-RR (same clone as 85063-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85063-4-RR (BAG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85063-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 85063-4-RR (same clone as 85063-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85063-4-RR (BAG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85063-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85063-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85063-4-RR against Human BAG2 were performed. The affinity constant is 0.644 nM.

BAG2 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 85063-4-PBS

Clone No.242717G4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

242717G4, BAG-2, KIAA0576

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Product Information

85063-4-PBS targets BAG2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag30873

  Product name: Recombinant human BAG2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-134 aa of NM_004282

  Sequence: MAQAKINAKANEGRFCRSSSMADRSSRLLESLDQLELRVEALREAATAVEQEKEILLEMIHSIQNSQDMRQISDGEREELNLTANRLMGRTLTVEVSVETIRNPQQQESLKHATRIIDEVVNKFLDDLGNAKSH

• Full Name: BCL2-associated athanogene 2
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_004282
• Gene Symbol: BAG2
• Gene ID (NCBI): 9532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95816
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BAG2 (BAG family molecular chaperone regulator 2) is one of six proteins in mammals that contain the BAG domain, which belongs to the BAG (Bcl-2-associated athanogene) family. BAG2 has been described as a negative regulator of the chaperone-associated ubiquitin ligase C terminus of Hsc70-interacting protein (CHIP) that participates in the ubiquitin-mediated proteasomal degradation of misfolded substrate proteins (PMID: 16207813). BAG2 is widely expressed in human tissues, including brown adipose, heart and lung tissue, as well as in various types of tumor cells, including renal cell carcinoma, glioblastoma and thyroid carcinoma cells (PMID: 28536620). BAG2 overexpression is associated with poor prognosis in patients and mutp53 accumulation in tumors (PMID: 26271008).