BAG2 Recombinant antibody

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  WB analysis using 85063-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85063-4-RR (BAG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85063-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85063-4-RR (BAG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 85063-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85063-4-RR against Human BAG2 were performed. The affinity constant is 0.644 nM.

BAG2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85063-4-RR

Clone No.242717G4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

242717G4, BAG-2, KIAA0576

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Tested Applications

• Positive WB detected in: A549 cells, U-87 MG cells, HeLa cells, HepG2 cells, U-251 cells, mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85063-4-RR targets BAG2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BAG2 fusion protein Ag30873
• Full Name: BCL2-associated athanogene 2
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_004282
• Gene Symbol: BAG2
• Gene ID (NCBI): 9532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95816
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAG2 (BAG family molecular chaperone regulator 2) is one of six proteins in mammals that contain the BAG domain, which belongs to the BAG (Bcl-2-associated athanogene) family. BAG2 has been described as a negative regulator of the chaperone-associated ubiquitin ligase C terminus of Hsc70-interacting protein (CHIP) that participates in the ubiquitin-mediated proteasomal degradation of misfolded substrate proteins (PMID: 16207813). BAG2 is widely expressed in human tissues, including brown adipose, heart and lung tissue, as well as in various types of tumor cells, including renal cell carcinoma, glioblastoma and thyroid carcinoma cells (PMID: 28536620). BAG2 overexpression is associated with poor prognosis in patients and mutp53 accumulation in tumors (PMID: 26271008).