• Featured Product
  • KD/KO Validated

BBS10 Polyclonal antibody

BBS10 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 12421-2-AP

Synonyms

Bardet Biedl syndrome 10, BBS10, C12orf58



Tested Applications

Positive WB detected inHepG2 cells, A549 cells, MCF-7 cells
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12421-2-AP targets BBS10 in WB, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBS10 fusion protein Ag3073
Full Name Bardet-Biedl syndrome 10
Calculated molecular weight 81 kDa
Observed molecular weight 81 kDa
GenBank accession numberBC013795
Gene symbol BBS10
Gene ID (NCBI) 79738
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID:20080638,19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).

Protocols

Product Specific Protocols
WB protocol for BBS10 antibody 12421-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cilia

Comparing the Bbs10 complete knockout phenotype with a specific renal epithelial knockout one highlights the link between renal defects and systemic inactivation in mice.

Authors - Noëlle Cognard
  • KO Validated
humanWB,IF

Proc Natl Acad Sci U S A

Transient ciliogenesis involving Bardet-Biedl syndrome proteins is a fundamental characteristic of adipogenic differentiation.

Authors - Marion Vincent V
  • KD Validated
human,mouseWB,IF

Proc Natl Acad Sci U S A

BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.

Authors - Seo Seongjin S