MKS1 Polyclonal antibody

MKS1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16206-1-AP

Synonyms

BBS13, Meckel syndrome type 1 protein, Meckel syndrome, type 1, MES, MKS, MKS1



Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells, HeLa cells, mouse uterus tissue, SH-SY5Y cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells and Mouse embryonic fibroblasts

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16206-1-AP targets MKS1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MKS1 fusion protein Ag9177
Full Name Meckel syndrome, type 1
Calculated molecular weight 559 aa, 65 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC010061
Gene symbol MKS1
Gene ID (NCBI) 54903
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MKS1 (Meckel syndrome type 1 protein) is a 559-amino acid protein that contains a conserved B9 domain. It is a component of a large protein complex which localizes to the ciliary transition zone and regulates mammalian ciliogenesis and ciliary membrane composition (PMID: 21725307). MKS1 is required for ciliary structure and function, and is involved in centrosome migration to the apical cell surface during early ciliogenesis (PMID: 17185389; 19515853). Broad tissue expression of the MKS1 gene has been reported (PMID: 16415886). Defects in MKS1 are the cause of Meckel syndrome type 1 (MKS1), an autosomal recessive lethal malformation syndrome characterized by renal cystic dysplasia, central nervous system malformations, and hepatic developmental defects (PMID: 16415886). In addition, defects in MKS1 are also the cause of Bardet-Biedl syndrome type 13 (BBS13) (PMID: 18327255).

Protocols

Product Specific Protocols
WB protocol for MKS1 antibody 16206-1-APDownload protocol
IHC protocol for MKS1 antibody 16206-1-APDownload protocol
IP protocol for MKS1 antibody 16206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nephrology (Carlton)

Visualizing renal primary cilia.

Authors - James A Deane
mouseIF

Dev Dyn

Fused (Stk36) is a ciliary protein required for central pair assembly and motile cilia orientation in the mammalian oviduct.

Authors - Nozawa Yoko Inès YI
humanWB

Sci Rep

Deacetylation of α-tubulin and cortactin is required for HDAC6 to trigger ciliary disassembly.

Authors - Jie Ran
humanIF

Biophys J

Super-Resolution Imaging Reveals TCTN2 Depletion-Induced IFT88 Lumen Leakage and Ciliary Weakening.

Authors - Rueyhung Roc Weng
humanIF

J Cell Sci

Glucose deprivation induces primary cilium formation through mTORC1 inactivation.

Authors - Kengo Takahashi
humanIF

Sci Rep

Superresolution Pattern Recognition Reveals the Architectural Map of the Ciliary Transition Zone.

Authors - T Tony Yang