BAP31 Polyclonal antibody

• Featured Product
• KD/KO Validated

BAP31 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (1)

Cat no : 11200-1-AP

Synonyms

6C6 AG, BAP31, BCAP31, CDM, DXS1357E, p28, Protein CDM

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Validation Data Gallery

• 

  WB analysis using 11200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11200-1-AP (BAP31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11200-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11200-1-AP (BAP31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11200-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11200-1-AP (BAP31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11200-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11200-1-AP (BAP31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11200-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11200-1-AP (BAP31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 11200-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 11200-1-AP (BAP31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 11200-1-AP

  IP result of anti-BAP31 (IP:11200-1-AP, 3ug; Detection:11200-1-AP 1:1000) with A431 cells lysate 3300ug.

• 

  IHC staining of human breast cancer using 11200-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11200-1-AP (BAP31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11200-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11200-1-AP (BAP31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11200-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11200-1-AP (BAP31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11200-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11200-1-AP (BAP31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11200-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BAP31 antibody (11200-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), phalloidine stains F-actin (red).

• 

  FC experiment of HeLa using 11200-1-AP

  1X10^6 HeLa cells were stained with 0.2ug BAP31 antibody (11200-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: A431 cells, human placenta tissue, HEK-293 cells, HeLa cells, mouse placenta tissue, MCF-7 cells, THP-1 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tisssue
• Positive IP detected in: A431 cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 16 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

11200-1-AP targets BAP31 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BAP31 fusion protein Ag1668
• Full Name: B-cell receptor-associated protein 31
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC014323
• Gene Symbol: BCAP31
• Gene ID (NCBI): 10134
• RRID: AB_2065142
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAP31 is a chaperone protein that is abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.

Publications

Species	Application	Title
	WB	Nat Biotechnol; Efficient proximity labeling in living cells and organisms with TurboID.; Authors - Tess C Branon
human	WB	Nat Methods; Directed evolution of APEX2 for electron microscopy and proximity labeling.; Authors - Stephanie S Lam
human	WB	Nat Commun; HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins.; Authors - Ulrike Zinnall
human	WB	EMBO J; FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.; Authors - Wenxian Wu
human	WB,IP	EMBO J; Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform for apoptosis induction.; Authors - Iwasawa Ryota R ; KD Validated
human,rat	WB	Food Chem Toxicol; Caffeic acid phenethyl ester protects against doxorubicin-induced cardiotoxicity and increases chemotherapeutic efficacy by regulating the unfolded protein response; Authors - Ying Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (11-02-2020)

This antibody labeled the ER area clearly

• Applications: Immunofluorescence

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