Validation Data Gallery
|Positive WB detected in||A431 cells, human placenta tissue, HEK-293 cells, HeLa cells, mouse placenta tissue, MCF-7 cells, THP-1 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tisssue|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human breast cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11200-1-AP targets BAP31 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BAP31 fusion protein Ag1668|
|Full Name||B-cell receptor-associated protein 31|
|Calculated molecular weight||28 kDa|
|Observed molecular weight||28 kDa|
|GenBank accession number||BC014323|
|Gene ID (NCBI)||10134|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
BAP31 is a chaperone protein that is abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.
|Product Specific Protocols|
|WB protocol for BAP31 antibody 11200-1-AP||Download protocol|
|IHC protocol for BAP31 antibody 11200-1-AP||Download protocol|
|IF protocol for BAP31 antibody 11200-1-AP||Download protocol|
|IP protocol for BAP31 antibody 11200-1-AP||Download protocol|
|FC protocol for BAP31 antibody 11200-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Efficient proximity labeling in living cells and organisms with TurboID.
Directed evolution of APEX2 for electron microscopy and proximity labeling.
HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins.
FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.
Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform for apoptosis induction.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (11-02-2020)
This antibody labeled the ER area clearly