Tested Applications
| Positive WB detected in | A431 cells, HEK-293 cells, HeLa cells, MCF-7 cells, THP-1 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tisssue |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human breast cancer tissue, human colon tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, U2OS cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11200-1-AP targets BAP31 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1668 Product name: Recombinant human BCAP31 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-246 aa of BC014323 Sequence: MSLQWTAVATFLYAEVFVVLLLCIPFISPKRWQKIFKSRLVELLVSYGNTFFVVLIVILVLLVIDAVREIREYDDVTEKVNLQNNPGAMEHFHMKLFRAQRNLYIAGFSLLLSFLLRRLVTLISQQATLLASNEAFKKQAESASEAAKKYMEENDQLKKGAAVDGGKLDVGNAEVKLEEENRSLKADLQKLKDELASTKQKLEKAENQVLAMRKQSEGLTKEYDRLLEEHAKLQAAVDGPMDKKEE Predict reactive species |
| Full Name | B-cell receptor-associated protein 31 |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC014323 |
| Gene Symbol | BAP31 |
| Gene ID (NCBI) | 10134 |
| RRID | AB_2065142 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51572 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BAP31, also known as BCAP31, is a chaperone protein abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded proteins, and their targeting to the ER-associated-degradation (ERAD). CDIP1 and BAP31 interact upon ER stress to regulate the mitochondrial apoptosis pathway(PMID: 24139803). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for BAP31 antibody 11200-1-AP | Download protocol |
| IF protocol for BAP31 antibody 11200-1-AP | Download protocol |
| IHC protocol for BAP31 antibody 11200-1-AP | Download protocol |
| IP protocol for BAP31 antibody 11200-1-AP | Download protocol |
| WB protocol for BAP31 antibody 11200-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BAP31 Polyclonal antibody (Cat# 11200-1-AP) has 37 citations published in journals including Science, Nature Biotechnology, Nature Methods, Nature Communications, EMBO Journal, Cell Death & Disease, Free Radical Biology and Medicine, Journal of Biological Chemistry, and International Journal of Molecular Sciences. Research fields encompass ER-mitochondria contact sites, endoplasmic reticulum stress, apoptosis, neurodegeneration, cancer biology, liver injury, cardiotoxicity, innate immunity, protein translation, lipid droplet biogenesis, and proximity labeling methodologies.
| Species | Application | Title |
|---|---|---|
Science Mammalian oocytes store mRNAs in a mitochondria-associated membraneless compartment | ||
Nat Commun HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins. | ||
Cell Death Dis BAP31 represses endoplasmic reticulum stress-mediated apoptosis and alleviates neurodegeneration in Parkinson's disease |
Reviews
What customers say
One reviewer (Boyan Zhang) gave a 5-star rating for this antibody used in immunofluorescence. They reported that it clearly labeled the ER area. The review was posted on November 2, 2020, using lot 00018537.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (11-02-2020) | This antibody labeled the ER area clearly
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