|Positive WB detected in||HEK-293 cells, A431 cells, HeLa cells, human placenta tissue, mouse placenta tissue|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human breast cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11200-1-AP targets BAP31 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BAP31 fusion protein Ag1668|
|Full Name||B-cell receptor-associated protein 31|
|Calculated molecular weight||28 kDa|
|Observed molecular weight||28 kDa|
|GenBank accession number||BC014323|
|Gene ID (NCBI)||10134|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BAP31 is a chaperone protein that is abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.
|Product Specific Protocols|
|WB protocol for BAP31 antibody 11200-1-AP||Download protocol|
|IHC protocol for BAP31 antibody 11200-1-AP||Download protocol|
|IF protocol for BAP31 antibody 11200-1-AP||Download protocol|
|IP protocol for BAP31 antibody 11200-1-AP||Download protocol|
|FC protocol for BAP31 antibody 11200-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Death Dis
MiR-451a suppressing BAP31 can inhibit proliferation and increase apoptosis through inducing ER stress in colorectal cancer.
Res Vet Sci
Proteomic analyses reveal that Orf virus induces the activation and maturation of mouse bone marrow-derived dendritic cells.
J Biol Chem
Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.
J Cell Sci
Expansion of DUB functionality by alternative isoforms: USP35, a case study.
Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform for apoptosis induction.
FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (11-02-2020)
This antibody labeled the ER area clearly