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BAP31 Polyclonal antibody
BAP31 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, FC, ELISA
Cat no : 11200-1-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, human placenta tissue, HEK-293 cells, HeLa cells, mouse placenta tissue, MCF-7 cells, THP-1 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tisssue|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human breast cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 4 publications below|
|WB||See 14 publications below|
|IF||See 4 publications below|
|IP||See 1 publications below|
11200-1-AP targets BAP31 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BAP31 fusion protein Ag1668|
|Full Name||B-cell receptor-associated protein 31|
|Calculated molecular weight||28 kDa|
|Observed molecular weight||28 kDa|
|GenBank accession number||BC014323|
|Gene ID (NCBI)||10134|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
BAP31 is a chaperone protein that is abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.
|Product Specific Protocols|
|WB protocol for BAP31 antibody 11200-1-AP||Download protocol|
|IHC protocol for BAP31 antibody 11200-1-AP||Download protocol|
|IF protocol for BAP31 antibody 11200-1-AP||Download protocol|
|IP protocol for BAP31 antibody 11200-1-AP||Download protocol|
|FC protocol for BAP31 antibody 11200-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Efficient proximity labeling in living cells and organisms with TurboID.
Directed evolution of APEX2 for electron microscopy and proximity labeling.
HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins.
FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.
Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform for apoptosis induction.
Food Chem Toxicol
Caffeic acid phenethyl ester protects against doxorubicin-induced cardiotoxicity and increases chemotherapeutic efficacy by regulating the unfolded protein response
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (11-02-2020)
This antibody labeled the ER area clearly