BCAT2 Polyclonal antibody

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  WB analysis using 16417-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16417-1-AP (BCAT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16417-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16417-1-AP (BCAT2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 16417-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16417-1-AP (BCAT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 16417-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16417-1-AP (BCAT2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16417-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16417-1-AP (BCAT2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16417-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16417-1-AP (BCAT2 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of C2C12 using 16417-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using BCAT2 antibody (16417-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of HepG2 using 16417-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug BCAT2 Polyclonal antibody (16417-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

• Featured Product
• KD/KO Validated

BCAT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 16417-1-AP

Publications(28)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Branched-chain-amino-acid aminotransferase, mitochondrial, EC:2.6.1.42, ECA40, Placental protein 18, PP18

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Tested Applications

• Positive WB detected in: Jurkat cells, L02 cells, Raji cells, RAW 264.7 cells, MCF-7 cells, MDA-MB-231 cells
• Positive IHC detected in: human skin tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C2C12 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 25 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

16417-1-AP targets BCAT2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9573

  Product name: Recombinant human BCAT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 41-392 aa of BC001900

  Sequence: MTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

• Full Name: branched chain aminotransferase 2, mitochondrial
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC001900
• Gene Symbol: BCAT2
• Gene ID (NCBI): 587
• RRID: AB_10792411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15382
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Nat Cancer; Integrative proteogenomic profiling of high-risk prostate cancer samples from Chinese patients indicates metabolic vulnerabilities and diagnostic biomarkers; Authors - Baijun Dong ; KD Validated
mouse	IHC	Nat Cell Biol; BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.; Authors - Jin-Tao Li
mouse,human	WB	Nat Commun; Glucose promotes cell growth by suppressing branched-chain amino acid degradation.; Authors - Dan Shao
human	WB,IHC	Basic Res Cardiol; Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1.; Authors - Qiong Lai
human	WB	Cancer Res; Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma.; Authors - Bo Zhang
mouse	WB	Diabetes; Transketolase Deficiency in Adipose Tissues Protects Mice From Diet-Induced Obesity by Promoting Lipolysis.; Authors - Na Tian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

Antibody works well when diluted in 1X TBST. Bands appeared at 40 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver tissue

FH

chao (Verified Customer) (01-30-2020)

Strong, clear band for heart lysates.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart