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BCAT2 Polyclonal antibody
BCAT2 Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Human and More (3)
WB, IHC, IF, FC, ELISA
Cat no : 16417-1-AP
Validation Data Gallery
|Positive WB detected in||HepG2 cells, MCF-7 cells, L02 cells, Jurkat cells, Raji cells|
|Positive IHC detected in||human skin tissue, human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 14 publications below|
|IHC||See 3 publications below|
|IF||See 1 publications below|
16417-1-AP targets BCAT2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BCAT2 fusion protein Ag9573|
|Full Name||branched chain aminotransferase 2, mitochondrial|
|Calculated molecular weight||44 kDa|
|Observed molecular weight||38-42 kDa|
|GenBank accession number||BC001900|
|Gene ID (NCBI)||587|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).
|Product Specific Protocols|
|WB protocol for BCAT2 antibody 16417-1-AP||Download protocol|
|IHC protocol for BCAT2 antibody 16417-1-AP||Download protocol|
|IF protocol for BCAT2 antibody 16417-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Nat Cell Biol
BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.
Glucose promotes cell growth by suppressing branched-chain amino acid degradation.
Basic Res Cardiol
Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1.
Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma.
Transketolase Deficiency in Adipose Tissues Protects Mice From Diet-Induced Obesity by Promoting Lipolysis.
Cell Mol Life Sci
Regulation of branched-chain amino acid metabolism by hypoxia-inducible factor in glioblastoma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
chao (Verified Customer) (01-30-2020)
Strong, clear band for heart lysates.