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BCAT2 Polyclonal antibody

BCAT2 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


Human and More (3)





Cat no : 16417-1-AP


BCAT2, ECA40, Placental protein 18, PP18

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, L02 cells, Jurkat cells, Raji cells
Positive IHC detected inhuman skin tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16417-1-AP targets BCAT2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCAT2 fusion protein Ag9573
Full Name branched chain aminotransferase 2, mitochondrial
Calculated molecular weight 44 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC001900
Gene symbol BCAT2
Gene ID (NCBI) 587
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).


Product Specific Protocols
WB protocol for BCAT2 antibody 16417-1-APDownload protocol
IHC protocol for BCAT2 antibody 16417-1-APDownload protocol
IF protocol for BCAT2 antibody 16417-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Cell Biol

BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.

Authors - Jin-Tao Li

Nat Commun

Glucose promotes cell growth by suppressing branched-chain amino acid degradation.

Authors - Dan Shao
humanWB, IHC

Basic Res Cardiol

Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1.

Authors - Qiong Lai

Cancer Res

Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma.

Authors - Bo Zhang


Transketolase Deficiency in Adipose Tissues Protects Mice From Diet-Induced Obesity by Promoting Lipolysis.

Authors - Na Tian

Cell Mol Life Sci

Regulation of branched-chain amino acid metabolism by hypoxia-inducible factor in glioblastoma.

Authors - Bo Zhang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


chao (Verified Customer) (01-30-2020)

Strong, clear band for heart lysates.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart