Tested Applications
| Positive WB detected in | HepG2 cells, MCF-7 cells, L02 cells, Jurkat cells, Raji cells |
| Positive IHC detected in | human skin tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | C2C12 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 24 publications below |
| IHC | See 4 publications below |
| IF | See 3 publications below |
Product Information
16417-1-AP targets BCAT2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BCAT2 fusion protein Ag9573 Predict reactive species |
| Full Name | branched chain aminotransferase 2, mitochondrial |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 38-42 kDa |
| GenBank Accession Number | BC001900 |
| Gene Symbol | BCAT2 |
| Gene ID (NCBI) | 587 |
| RRID | AB_10792411 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15382 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BCAT2 antibody 16417-1-AP | Download protocol |
| IHC protocol for BCAT2 antibody 16417-1-AP | Download protocol |
| IF protocol for BCAT2 antibody 16417-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cancer Integrative proteogenomic profiling of high-risk prostate cancer samples from Chinese patients indicates metabolic vulnerabilities and diagnostic biomarkers
| ||
Nat Cell Biol BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma. | ||
Nat Commun Glucose promotes cell growth by suppressing branched-chain amino acid degradation. | ||
Basic Res Cardiol Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1. | ||
Cancer Res Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma. | ||
Diabetes Transketolase Deficiency in Adipose Tissues Protects Mice From Diet-Induced Obesity by Promoting Lipolysis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kamal (Verified Customer) (02-15-2024) | Antibody works well when diluted in 1X TBST. Bands appeared at 40 kDa.
|
FH chao (Verified Customer) (01-30-2020) | Strong, clear band for heart lysates.
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