BCKDHA Recombinant antibody, PBS Only (Capture/Detector)

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  Sandwich ELISA standard curve of MP02125-3

  Sandwich ELISA standard curve of MP02125-3, Human BCKDHA Recombinant Matched Antibody Pair - PBS only. 85799-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag32613. 85799-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP02125-3

  The mean BCKDHA concentration was determined to be 33.1 ng/mL in A549 cell extract based on a 1.3 mg/mL extract load, 11.9 ng/mL in U-87 MG cell extract based on a 2.0 mg/mL extract load and 69.3 ng/mL in THP-1 cell extract based on a 1.5 mg/mL extract load.

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  Cytometric bead array standard curve of MP02125-2

  Cytometric bead array standard curve of MP02125-2, BCKDHA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85799-3-PBS. Detection antibody: 85799-1-PBS. Standard: Ag32613. Range: 0.625-80 ng/mL.

BCKDHA Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85799-1-PBS

Clone No.250051H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial, BCKDH E1-alpha, Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, EC:1.2.4.4, MSU

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Product Information

85799-1-PBS targets BCKDHA as part of a matched antibody pair:

MP02125-2: 85799-3-PBS capture and 85799-1-PBS detection (validated in Cytometric bead array)

MP02125-3: 85799-1-PBS capture and 85799-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BCKDHA fusion protein Ag32613
• Full Name: branched chain keto acid dehydrogenase E1, alpha polypeptide
• Calculated Molecular Weight: 50 kDa
• GenBank Accession Number: BC008933
• Gene Symbol: BCKDHA
• Gene ID (NCBI): 593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12694
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.