Product Information
68596-1-PBS targets BCKDHB as part of a matched antibody pair:
MP50570-1: 68596-2-PBS capture and 68596-1-PBS detection (validated in Cytometric bead array)
MP50570-3: 68596-4-PBS capture and 68596-1-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33802 Product name: Recombinant human BCKDHB protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-392 aa of BC040139 Sequence: Predict reactive species |
| Full Name | branched chain keto acid dehydrogenase E1, beta polypeptide |
| Calculated Molecular Weight | 392 aa, 43 kDa |
| Observed Molecular Weight | 35-37 kDa |
| GenBank Accession Number | BC040139 |
| Gene Symbol | BCKDHB |
| Gene ID (NCBI) | 594 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P21953 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
Branched-chain alpha-keto acid dehydrogenase E1 component beta chain (BCKDHB; BCKDE1B; BCKDH E1-beta) is a subunit of the BCKDH complex, which is a mitochondrial enzyme in the degradation pathway for branched-chain amino acids (BCAA). Together with BCKDHA, BCKDHB forms the E1 subunit of this complex, whereas DBT and DLD are the E2 and E3 subunits, respectively. A deficiency of the BCKDH complex in humans causes maple syrup urine disease (MSUD), a severe neurometabolic disorder diagnosed by the detection alloisoleucine in plasma (MIM 248600). (PMID: 30709776)
