BCL11B Polyclonal antibody

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  WB analysis of Jurkat using 55414-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 55414-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 55414-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 55414-1-AP

  IP result of anti-BCL11B (IP:55414-1-AP, 4ug; Detection:55414-1-AP 1:5000) with Jurkat cells lysate 1320 ug.

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  IHC staining of mouse brain using 55414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55414-1-AP (BCL11B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 55414-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 55414-1-AP (BCL11B antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

BCL11B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 55414-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

COUP TF interacting protein 2, BCL-11B, B-cell lymphoma/leukemia 11B, B-cell CLL/lymphoma 11B, B cell lymphoma/leukemia 11B

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse thymus tissue, MOLT-4 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

55414-1-AP targets BCL11B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: B-cell CLL/lymphoma 11B (zinc finger protein)
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: NM_022898
• Gene Symbol: BCL11B
• Gene ID (NCBI): 64919
• RRID: AB_11182609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9C0K0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL11B, also named CTIP2, RIT1, CTIP-2, ZNF856B, and hRIT1-alpha, is a tumor-suppressor protein involved in T-cell lymphomas. It may function on the P53-signaling pathway. BCL11B is a key regulator of both differentiation and survival during thymocyte development. It depresses transcription through direct, TFCOUP2-independent binding to a GC-rich response element. This antibody is specific to BCL11B. The calculated molecular weight of BCL11B is 95 kDa but the modified BCL11B protein is about 116 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
Mouse		Cell Rep; XLF/Cernunnos loss impairs mouse brain development by altering symmetric proliferative divisions of neural progenitors; Authors - Amandine Bery
human	WB	Arch Virol; Increased expression of BCL11B and its recruited chromatin remodeling factors during highly active antiretroviral therapy synergistically represses the transcription of human immunodeficiency virus type 1 and is associated with residual immune activation.; Authors - Juan Wang
mouse	WB,IF	EMBO J; BMP suppresses Wnt signaling via the Bcl11b-regulated NuRD complex to maintain intestinal stem cells; Authors - Yehua Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (12-09-2023)

Good for ICC on subcerebral neurons

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: ROs