• Featured Product
  • KD/KO Validated

BCL2 Monoclonal antibody

BCL2 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

4H8C6

Cat no : 60178-1-Ig

Synonyms

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, BCL-2, Oncogene B cell leukemia 2



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, pig spleen tissue, U-937 cells
Positive IP detected inU-937 cells
Positive IHC detected inhuman appendicitis tissue, human tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:6400-1:25600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60178-1-Ig targets BCL2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivity human, pig, rat, swine
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BCL2 fusion protein Ag3508
Full Name B-cell CLL/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC027258
Gene symbol BCL2
Gene ID (NCBI) 596
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

1) What is Bcl-2?
Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.
2) FAQs for Bcl-2

A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.

Protocols

Product Specific Protocols
WB protocol for BCL2 antibody 60178-1-IgDownload protocol
IHC protocol for BCL2 antibody 60178-1-IgDownload protocol
IF protocol for BCL2 antibody 60178-1-IgDownload protocol
IP protocol for BCL2 antibody 60178-1-IgDownload protocol
FC protocol for BCL2 antibody 60178-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biol Res

Derlin1 functions as an oncogene in cervical cancer via AKT/mTOR signaling pathway.

Authors - Ling Li
mouseWB

Stress

Regulating glycolysis, the TLR4 signal pathway and expression of RBM3 in mouse liver in response to acute cold exposure.

Authors - Hongzhao Shi
humanWB

Mol Cell Probes

CDKL1 promotes the chemoresistance of human oral squamous cell carcinoma cells to hydroxycamptothecin.

Authors - Keyi Li
mouseWB

Int J Mol Sci

Effects of Cold-inducible RNA-binding Protein (CIRP) on Liver Glycolysis during Acute Cold Exposure in C57BL/6 Mice.

Authors - Peng Liu
humanWB

Exp Cell Res

Rho GDP dissociation inhibitor α silencing attenuates silicosis by inhibiting RhoA/Rho kinase signalling.

Authors - Zhongqiu Wei
swineWB

Biosci Rep

Resveratrol attenuates mechanical compression-induced nucleus pulposus cell apoptosis through regulating the ERK1/2 signaling pathway in a disc organ culture.

Authors - Feng Wen