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BCL2 Monoclonal antibody
BCL2 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 60178-1-Ig
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HepG2 cells, Jurkat cells, pig spleen tissue, U-937 cells|
|Positive IP detected in||U-937 cells|
|Positive IHC detected in||human appendicitis tissue, human tonsillitis tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, human tonsillitis tissue|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:6400-1:25600|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
60178-1-Ig targets BCL2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, pig samples.
|Tested Reactivity||human, pig|
|Cited Reactivity||human, rat, swine|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||BCL2 fusion protein Ag3508|
|Full Name||B-cell CLL/lymphoma 2|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||26 kDa|
|GenBank accession number||BC027258|
|Gene ID (NCBI)||596|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
1) What is Bcl-2?Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.
2) FAQs for Bcl-2
A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.
|Product Specific Protocols|
|WB protocol for BCL2 antibody 60178-1-Ig||Download protocol|
|IHC protocol for BCL2 antibody 60178-1-Ig||Download protocol|
|IF protocol for BCL2 antibody 60178-1-Ig||Download protocol|
|IP protocol for BCL2 antibody 60178-1-Ig||Download protocol|
|FC protocol for BCL2 antibody 60178-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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