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BCL2 Monoclonal antibody

BCL2 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig and More (1)

Applications

WB, IP, IHC, IF, ICC, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

4H8C6

Cat no : 60178-1-Ig

Synonyms

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, BCL-2, Oncogene B cell leukemia 2



Tested Applications

Positive WB detected inU2OS cells, HEK-293 cells, HepG2 cells, Jurkat cells, MCF-7 cells, pig spleen tissue, THP-1 cells, U-937 cells, MOLT-4 cells, HL-60 cells, HT-1080 cells, Raji cells
Positive IP detected inU-937 cells
Positive IHC detected inhuman appendicitis tissue, human tonsillitis tissue, human lymphoma tissue, Jurkat cells
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, human tonsillitis tissue, human appendicitis tissue
Positive FC detected inhuman peripheral blood lymphocytes, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:6400-1:25600
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

60178-1-Ig targets BCL2 in WB, IP, IHC, IF, ICC, FC, CoIP, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman, monkey, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BCL2 fusion protein Ag3508
Full Name B-cell CLL/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC027258
Gene symbol BCL2
Gene ID (NCBI) 596
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

1) What is Bcl-2?
Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.
2) FAQs for Bcl-2

A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.

Protocols

Product Specific Protocols
IHC protocol for BCL2 antibody 60178-1-IgDownload protocol
IF protocol for BCL2 antibody 60178-1-IgDownload protocol
IP protocol for BCL2 antibody 60178-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Extracell Vesicles

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

Authors - Xiaohan Gao
ratWB

Redox Biol

Heat shock protein 22 modulates NRF1/TFAM-dependent mitochondrial biogenesis and DRP1-sparked mitochondrial apoptosis through AMPK-PGC1α signaling pathway to alleviate the early brain injury of subarachnoid hemorrhage in rats.

Authors - Haiyan Fan
humanWB

Redox Biol

Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.

Authors - Zhongren Xu
humanWB

J Immunother Cancer

Switch receptor T3/28 improves long-term persistence and antitumor efficacy of CAR-T cells

Authors - Songbo Zhao
monkeyCoIP

Autophagy

Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress

Authors - Jia-min Yan
humanWB

Autophagy

TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer.

Authors - Cefan Zhou