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BCL2 Antibody 15 Publications

Mouse Monoclonal| Catalog number: 60178-1-Ig |CloneNo.: 4H8C6

Featured Product KD/KO validated

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, CoIP, ELISA
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Images See all

BCL2 Antibody WB HEK-293 cells 60178-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 60178-1-Ig (BCL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

BCL2 Antibody IHC human appendicitis tissue 60178-1-Ig

Immunohistochemistry of paraffin-embedded human appendicitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

BCL2 Antibody IHC human appendicitis tissue 60178-1-Ig

Immunohistochemistry of paraffin-embedded human appendicitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

BCL2 Antibody WB Jurkat cells 60178-1-Ig

WB result of BCL2 antibody (60178-1-Ig, 1:1000) with si-control and si-BCL2 transfected Jurkat cells.

BCL2 Antibody IP U-937 cells 60178-1-Ig

IP Result of anti-BCL2 (IP:60178-1-Ig, 5ug; Detection:60178-1-Ig 1:1000) with U-937 cells lysate 4000ug.

BCL2 Antibody IF MCF-7 cells 60178-1-Ig

Immunofluorescent analysis of ( -20°C Ethanol) fixed MCF-7 cells using 60178-1-Ig (BCL2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

BCL2 Antibody FC Jurkat cells 60178-1-Ig

1X10^6 Jurkat cells were stained with 0.2ug BCL2 antibody (60178-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

Species specificity:
human, pig, swine
Positive WB detected in:
HEK-293 cells, HepG2 cells, Jurkat cells, pig spleen tissue, U-937 cells
Positive IP detected in:
U-937 cells
Positive IHC detected in:
human appendicitis tissue, human tonsillitis tissue, human lymphoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells
Positive FC detected in:
Jurkat cells
Recommended dilution:
WB : 1:2000-1:10000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:6400-1:25600
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
BCL2 fusion protein Ag3508
Full name:
B-cell CLL/lymphoma 2
Calculated molecular weight:
26 kDa
Observed molecular weight:
26 kDa
GenBank accession number:
BC027258
Gene ID (NCBI):
596
Gene symbol
BCL2
Synonyms
Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, BCL-2, Oncogene B cell leukemia 2

Background

1) What is Bcl-2?
Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.
2) FAQs for Bcl-2

A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.


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