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- KD/KO Validated
BCL2 Monoclonal antibody
BCL2 Monoclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig and More (1)
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
4H8C6
Cat no : 60178-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | U2OS cells, U-937 cells, Jurkat cells, pig spleen tissue, THP-1 cells, HepG2 cells, HEK-293 cells, HT-1080 cells, MCF-7 cells, Raji cells |
| Positive IP detected in | U-937 cells |
| Positive IHC detected in | human appendicitis tissue, human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells, human tonsillitis tissue |
| Positive FC detected in | human peripheral blood lymphocytes, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:6400-1:25600 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 91 publications below |
| IHC | See 5 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
60178-1-Ig targets BCL2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, pig, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | BCL2 fusion protein Ag3508 |
| Full Name | B-cell CLL/lymphoma 2 |
| Calculated molecular weight | 26 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC027258 |
| Gene symbol | BCL2 |
| Gene ID (NCBI) | 596 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
1) What is Bcl-2?
Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.2) FAQs for Bcl-2
A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BCL2 antibody 60178-1-Ig | Download protocol |
| IHC protocol for BCL2 antibody 60178-1-Ig | Download protocol |
| IF protocol for BCL2 antibody 60178-1-Ig | Download protocol |
| IP protocol for BCL2 antibody 60178-1-Ig | Download protocol |
| FC protocol for BCL2 antibody 60178-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biol Res Derlin1 functions as an oncogene in cervical cancer via AKT/mTOR signaling pathway. | ||
Stress Regulating glycolysis, the TLR4 signal pathway and expression of RBM3 in mouse liver in response to acute cold exposure. | ||
Mol Cell Probes CDKL1 promotes the chemoresistance of human oral squamous cell carcinoma cells to hydroxycamptothecin. | ||
Int J Mol Sci Effects of Cold-inducible RNA-binding Protein (CIRP) on Liver Glycolysis during Acute Cold Exposure in C57BL/6 Mice. | ||
Exp Cell Res Rho GDP dissociation inhibitor α silencing attenuates silicosis by inhibiting RhoA/Rho kinase signalling. | ||
Biosci Rep Resveratrol attenuates mechanical compression-induced nucleus pulposus cell apoptosis through regulating the ERK1/2 signaling pathway in a disc organ culture. |
