CoraLite®555-conjugated BCL2 Monoclonal antibody

• Featured Product
• KD/KO Validated

BCL2 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

4H8C6

Cat no : CL555-60178

Synonyms

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, Oncogene B cell leukemia 2

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Validation Data Gallery

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  IF Staining of human appendicitis using CL555-60178

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®555 BCL2 antibody (CL555-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  IF Staining of human appendicitis using CL555-60178

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®555 BCL2 antibody (CL555-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  IF Staining of NIH/3T3 using CL555-60178

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite®555 BCL2 antibody (CL555-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  IF Staining of MCF-7 using CL555-60178

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®555 BCL2 antibody (CL555-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  FC experiment of HeLa using CL555-60178

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human BCL2 (CL555-60178, Clone:4H8C6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: human appendicitis tissue, NIH/3T3 cells, MCF-7 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-60178 targets BCL2 in IF, FC (Intra) applications and shows reactivity with human, pig, mouse samples.

• Tested Reactivity: human, pig, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: BCL2 fusion protein Ag3508
• Full Name: B-cell CLL/lymphoma 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC027258
• Gene Symbol: BCL2
• Gene ID (NCBI): 596
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

1) What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2) FAQs for Bcl-2

A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.