CoraLite® Plus 647-conjugated BCL2 Monoclonal antibody

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  IF Staining of MCF-7 using CL647-60178

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 647 BCL2 antibody (CL647-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  IF Staining of HeLa using CL647-60178

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647 BCL2 antibody (CL647-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  FC experiment of human peripheral blood lymphocytes using CL647-60178

  1x10^6 human peripheral blood lymphocytes were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated BCL2 Monoclonal antibody (CL647-60178, Clone:4H8C6)(red), or 0.4 ug CoraLite® Plus 647 Mouse IgG2b Isotype Control (MPC-11) (CL647-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

BCL2 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL647-60178

Clone No.4H8C6

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF/ICC, FC (Intra)

Synonyms

BCL-2, 4H8C6

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells, HeLa cells
• Positive FC (Intra) detected in: human peripheral blood lymphocytes

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-60178 targets BCL2 in IF/ICC, FC (Intra) applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: BCL2 fusion protein Ag3508
• Full Name: B-cell CLL/lymphoma 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC027258
• Gene Symbol: BCL2
• Gene ID (NCBI): 596
• RRID: AB_2934981
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10415
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

1) What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2) FAQs for Bcl-2

A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.