CoraLite® Plus 647-conjugated BCL2 Monoclonal antibody

• Featured Product
• KD/KO Validated

BCL2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4H8C6

Cat no : CL647-60178

Synonyms

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, Oncogene B cell leukemia 2

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Validation Data Gallery

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  FC experiment of Jurkat using CL647-60178

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human BCL2 (CL647-60178, Clone:4H8C6) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: Jurkat cells

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Product Information

CL647-60178 targets BCL2 in FC (Intra) applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: BCL2 fusion protein Ag3508
• Full Name: B-cell CLL/lymphoma 2
• Calculated molecular weight: 26 kDa
• Observed molecular weight: 26 kDa
• GenBank accession number: BC027258
• Gene symbol: BCL2
• Gene ID (NCBI): 596
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

1) What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2) FAQs for Bcl-2

A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.